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Characteristics
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The FITC anti-Rainbow trout Ig monoclonal antibody can be used to directly detect a generalized or pathogen-specific increase in immunoglobulin level. This monoclonal antibody only recognizes the immunoglobulin heavy chain found in Oncorhynchus species (ie. Coho, Chum, Sockeye and Chinook) but not the Atlantic salmon heavy chain. This antibody can be used as a detection reagent for fish infection by either detecting a generalized increase in fish immunoglobulin level or by detecting specific antibody. For specific antibody detection, known infectious agents are used as the antigen in an ELISA assay, followed by samples of infected fish serum and then the anti-Rainbow Trout Ig monoclonal antibody. See ELISA protocol below. Specific uses for this monoclonal antibody include ELISA, Western blots and immunofluorescence (ie. FACS).
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