Western Blot of Rabbit anti-Human Fc antibody. Lane 1: Human Fc. Lane 2: none. Load: 100 ng per lane. Primary antibody: Human Fc antibody at 1:1,000 for overnight at 4°C. Secondary antibody: DyLight™ 649 rabbit secondary antibody at 1:20,000 for 30 min at RT. Block: MB-070 for 30 min at RT. Predicted/Observed size: 28 kDa, 28 kDa for Human Fc. Other band(s): Human Fc splice variants and isoforms.