Western Blot of Rabbit anti-Chicken IgG Antibody. Lane 1: Chicken IgG. Lane 2: None. Load: 100 ng per lane. Primary antibody: Chicken IgG antibody at 1:1000 for overnight at 4°C. Secondary antibody: DyLight™ 649 rabbit secondary antibody at 1:20,000 for 30 min at RT. Block: 5% BLOTTO 30 min at RT. Predicted/Observed size: 72 and 28 kDa, 72 and 28 kDa for Chicken IgG. Other band(s): none.