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  • 产品名称：Goatanti-RabbitIgG(Heavy&LightChain)Antibody(HRP)

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  • 产品厂商：PromoCell
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简单介绍：
Goatanti-RabbitIgG(Heavy&LightChain)Antibody(HRP)
详情介绍：
Immunogen Rabbit IgG, whole molecule
Isotype IgG
Characteristics Peroxidase Conjugated Affinity Purified anti-Rabbit IgG [H&L] [Goat]
Conjugate: Horseradish Peroxidase (HRP)
Purification Peroxidase Conjugated Affinity Purified anti-Rabbit IgG was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
Research Area Immunology, Secondary Antibodies
Application Notes Peroxidase Conjugated Affinity Purified anti-Rabbit IgG is suitable for immunoblotting (Western or dot blot), ELISA, immunoperoxidase electron microscopy and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency. Recommended dilutions are as follows, ELISA 1:20,000 - 1:100,000, Western Blot 1:2,000 - 1:10,000, Immunohistochemistry 1:1,000 - 1:5,000. (Optimal dilution should be determined by user.).
Restrictions For Research Use only
Format Lyophilized
Reconstitution Restore with 1.0 mL of deionized water (or equivalent).
Concentration 2.0 mg/mL
Buffer STABILIZER: 10 mg/mL BSA, IgG and Protease free, PRESERVATIVE: 0.01 % (w/v) Gentamicin Sulfate
Preservative Gentamicin sulfate
Handling Advice Avoid repeated freezing and thawing. Dilute only prior to immediate use
Centrifuge product if not completely clear after standing at room temperature.
Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.
Storage 4 °C
Storage Comment Peroxidase Conjugated Affinity Purified anti-Rabbit IgG can be stored vial at 4 °C prior to restoration.
This product is stable for several weeks at 4 °C as an undiluted liquid. For extended storage aliquot contents and freeze at -24 °C or below.
Expiry Date 12 months
Background publications Farr, Nakane: "Immunohistochemistry with enzyme labeled antibodies: a brief review." in: Journal of immunological methods, Vol. 47, Issue 2, pp. 129-44, 1982 (PubMed).