Western Blot of Goat anti-F(ab')2 Rabbit IgG F(ab')2 Antibody Peroxidase Conjugated Pre-Adsorbed. Lane 1: Rabbit IgG F(ab')2. Lane 2: none. Load: 100 ng per lane. Secondary antibody: F(ab')2 Rabbit IgG F(ab')2 Antibody Peroxidase Conjugated Pre-Adsorbed at 1:1,000 for 60 min at RT. Block: MB-070 for 30 min at RT. Predicted/Observed size: 28 kDa, 28 kDa for Rabbit IgG F(ab')2.