Western Blot of Goat anti-F(ab')2 Rabbit IgG F(c) Antibody Biotin Conjugated Lane 1: Rabbit Fc Load: 100 ng per lane Primary antibody: F(ab')2 Rabbit IgG F(c) Antibody Biotin Conjugated at 1:1000 for overnight at 4°C Secondary antibody: HRP Streptavidin at 1:40,000 for 30 min at RT Block: MB-070 for 30 min RT Predicted/Observed size: 28 kDa, 28 kDa