Western Blot of Donkey anti-Mouse IgG (H&L) Antibody Peroxidase Conjugated Lane 1: Mouse IgG Load: 50 ng per lane Secondary antibody: Mouse IgG (H&L) Antibody Peroxidase Conjugated at 1:1,000 for 60 min at RT Block: MB-070 for 30 min at RT Predicted/Observed size: 55 and 28 kDa, 55 and 28 kDa for Mouse IgG