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  • 产品名称：Mouseanti-HumanIgEAntibody(PE)

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  • 产品厂商：BioLeaf
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简单介绍：
Mouseanti-HumanIgEAntibody(PE)
详情介绍：
Immunogen Purified human IgE.
Clone BE5
Isotype IgG1
Specificity The antibody BE5 reacts with human IgE, it recognizes an epitope different from the ones recognized by 4G7 and 4H10 antibodies to IgE.
Characteristics The purified antibody is conjugated with R-Phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography.
Background Immunoglobulin E (IgE) is a 180 kDa soluble protein serving as an antigen-specific unit of mast cell effector mechanisms. IgE has the lowest serum concentration of all immunoglobulins (approximately 0.5 mg/l) in healthy individuals, but upon allergen challenge its concentration in blood increases dramatically. Although biological survival of free IgE is very short (T1/2 = 2 days), it is stabilized after binding to its high affinity receptor. Unlike IgM- IgG- and IgA-committed B cells, IgE-switched B cells do not undergo clonal expansion.
Molecular Weight 180 kDa
Research Area Immunology, Secondary Antibodies
Application Notes The reagent is designed for Flow Cytometry analysis. Suggested working dilution is 5 µ,g/mL. Indicated dilution is recommended starting point for use of this product. Working concentrations should be determined by the investigator.
Restrictions For Research Use only
Format Liquid
Concentration 0.1 mg/mL
Buffer The reagent is provided in phosphate buffered saline (PBS) containing 15 mM sodium azide and 0.2 % (w/v) high-grade protease free Bovine Serum Albumin (BSA) as a stabilizing agent.
Handling Advice Do not freeze.
Avoid prolonged exposure to light.
Storage 4 °C
Storage Comment Store in the dark at 2-8°C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.
Product cited in: Kaulfürst-Soboll, Mertens, Brehler, von Schaewen: "Reduction of cross-reactive carbohydrate determinants in plant foodstuff: elucidation of clinical relevance and implications for allergy diagnosis." in: PLoS ONE, Vol. 6, Issue 3, pp. e17800, 2011 (PubMed).

Background publications Gould, Beavil, Vercelli: "IgE isotype determination: epsilon-germline gene transcription, DNA recombination and B-cell differentiation." in: British medical bulletin, Vol. 56, Issue 4, pp. 908-24, 2001 (PubMed).

Brinkmann, Heusser: "T cell-dependent differentiation of human B cells into IgM, IgG, IgA, or IgE plasma cells: high rate of antibody production by IgE plasma cells, but limited clonal expansion of IgE precursors." in: Cellular immunology, Vol. 152, Issue 2, pp. 323-32, 1994 (PubMed).

Balogh, Merétey, Falus, Bozsóky: "Serological abnormalities in juvenile chronic arthritis: a review of 46 cases." in: Annals of the rheumatic diseases, Vol. 39, Issue 2, pp. 129-34, 1980 (PubMed).

Franklin: "Structure and function of immunoglobulins." in: Acta endocrinologica. Supplementum, Vol. 194, pp. 77-95, 1975 (PubMed).

Fuller, Keyser: "Serum immunoglobulins after surgical operation." in: Clinical chemistry, Vol. 21, Issue 6, pp. 667-71, 1975 (PubMed).