Western Blot of Chicken anti-Human IgG Antibody Alkaline Phosphatase Conjugated Lane 1: Human IgG Load: 100 ng per lane Secondary antibody: Human IgG (H&L) Antibody Alkaline Phosphatase Conjugated at 1:1,000 for 60 min at RT Block: MB-070 for 30 min at RT Predicted/Observed size: 55 and 28 kDa, 55 kDa