Western Blot of Goat anti-Rabbit IgG Antibody Alkaline Phosphatase Conjugated. Lane 1: Rabbit IgG. Lane 2: None. Load: 100 ng per lane. Primary Antibody: None. Secondary antibody: Alkaline Phosphatase goat secondary antibody at 1:1,000 for 60 min at RT. Block: MB-070 for 30 min at RT. Predicted/Observed size: 55 and 28 kDa, 55 kDa for Rabbit IgG. Other Band(s): None.