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  • 产品名称：Mouseanti-HumanIgEAntibody

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  • 产品厂商：BioLeaf
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简单介绍：
Mouseanti-HumanIgEAntibody
详情介绍：
Immunogen Purified human IgE.
Clone 4H10
Isotype IgG1
Specificity The antibody 4H10 reacts with human IgE, it recognizes an epitope different from the ones recognized by BE5 and 4G7 antibodies to IgE.
Purification Purified from ascites by precipitation methods and ion exchange chromatography.
Purity > 95 % (by SDS-PAGE)
Background Immunoglobulin E (IgE) is a 180 kDa soluble protein serving as an antigen-specific unit of mast cell effector mechanisms. IgE has the lowest serum concentration of all immunoglobulins (approximately 0.5 mg/l) in healthy individuals, but upon allergen challenge its concentration in blood increases dramatically. Although biological survival of free IgE is very short (T1/2 = 2 days), it is stabilized after binding to its high affinity receptor. Unlike IgM- IgG- and IgA-committed B cells, IgE-switched B cells do not undergo clonal expansion.
Molecular Weight 180 kDa
Research Area Immunology, Secondary Antibodies
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Format Liquid
Concentration 1 mg/mL
Buffer Tris buffered saline (TBS) with 15 mM sodium azide, approx. pH 8.0
Handling Advice Do not freeze.
Storage 4 °C
Storage Comment Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label.
Background publications Gould, Beavil, Vercelli: "IgE isotype determination: epsilon-germline gene transcription, DNA recombination and B-cell differentiation." in: British medical bulletin, Vol. 56, Issue 4, pp. 908-24, 2001 (PubMed).

Brinkmann, Heusser: "T cell-dependent differentiation of human B cells into IgM, IgG, IgA, or IgE plasma cells: high rate of antibody production by IgE plasma cells, but limited clonal expansion of IgE precursors." in: Cellular immunology, Vol. 152, Issue 2, pp. 323-32, 1994 (PubMed).

Balogh, Merétey, Falus, Bozsóky: "Serological abnormalities in juvenile chronic arthritis: a review of 46 cases." in: Annals of the rheumatic diseases, Vol. 39, Issue 2, pp. 129-34, 1980 (PubMed).

Franklin: "Structure and function of immunoglobulins." in: Acta endocrinologica. Supplementum, Vol. 194, pp. 77-95, 1975 (PubMed).

Fuller, Keyser: "Serum immunoglobulins after surgical operation." in: Clinical chemistry, Vol. 21, Issue 6, pp. 667-71, 1975 (PubMed).