Western Blot of Rabbit anti-Human IgG (H&L) Antibody Biotin Conjugated Lane 1: Human IgG Load: 50 ng per lane Primary antibody: Human IgG (H&L) Antibody Biotin Conjugated at 1:1000 for 60 min RT Secondary antibody: HRP Conjugated Streptavidin at 1:40,000 for 30 min at RT Block: MB-070 for 30 min at RT