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Format
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Lyophilized
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Reconstitution
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Reconstitute to 0.5 mL with distilled water.
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Buffer
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Lyophilized from 0.01 M Tris-HCl, 0.21 M NaCl, pH 8.0 containing 15 mg/mL BSA, 0.05 % Sodium Azide
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Preservative
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Sodium azide
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Precaution of Use
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WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
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Handling Advice
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Do not freeze! Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
Do not add Sodium azide.
Each reagent is stable for the period shown on the bottle label if stored as directed.
Prepare working dilution only prior to immediate use. Please note that the concentration of protein and buffer salts will decrease to one-half of the original after the addition of glycerol. Centrifuge product if it is not completely clear after standing for 1-2 hours at room temperature. To judge clarity, draw product into a pasteur pipette.
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Storage
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4 °C
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Storage Comment
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Store lyophilized product at 2-8 °C. After reconstitution, product is stable for several weeks at 2-8 °C as an undiluted liquid. For extended storage after reconstitution, add an equal volume of glycerol (ACS or better grade) to make a final glycerol concentration of 50 % and store at -28 °C with or without aliquoting.
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Expiry Date
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12 months
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