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  • 产品名称：Goatanti-Mouse(Murine)IgG(Heavy&LightChain)Antibody(PE)

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  • 产品厂商：BioLeaf
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简单介绍：
Goatanti-Mouse(Murine)IgG(Heavy&LightChain)Antibody(PE)
详情介绍：
Immunogen F(ab')2 anti-Mouse IgG (H&L) was produced by repeated immunization with Mouse IgG whole molecule in goat.
Fragment F(ab')2 fragment
Specificity F(ab')2 Anti-MOUSE IgG (H&L) (GOAT) Antibody
Characteristics F(ab')2 anti-Mouse IgG (H&L) antibody generated in goat detects specifically Mouse IgG whole molecule. This secondary antibody anti-Mouse is ideal for investigators who routinely perform titration assays, western-blot, immunoprecipitation and more generally immunoassays.
The antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation. F(ab')2 Anti-MOUSE IgG (H&L) (GOAT) Antibody assays by immunoelectrophoresis resulted in a single precipitin arc against anti-Phycoerythrin, anti-Goat Serum, Mouse IgG and Mouse Serum.
Purification Immunoaffinity Chromatography.
Background Synonyms: Mouse F(ab')2 IgG (H&L) Antibody, Gt-a-Mouse IgG (H&L), F(ab')2 Mouse IgG (H&L) Antibody in goat, Mouse IgG (H&L) Secondary Antibody.
Research Area Immunology, Secondary Antibodies
Application Notes F(ab') 2 anti-Mouse IgG (H&L) antibody is suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity. The maximum amount of reagent required to stain 1 x 10^6 cells in flow cytometry is approximately 1.0 µg of antibody conjugate. Lesser amounts of reagent may be sufficient for staining. Optimal titers for other applications should be determined by the researcher. As a general guideline dilutions of 1:100 to 1:250 should be suitable for most applications.
Restrictions For Research Use only
Format Lyophilized
Reconstitution Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 1.0 mL
Concentration 0.5 mg/mL
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Product is photosensitive and should be protected from light.
Avoid cycles of freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store vial at 4 °C prior to restoration. For extended storage aliquot contents and freeze at -24 °C or below. This product is stable for several weeks at 4 °C as an undiluted liquid.
Expiry Date 12 months
Supplier Images
Western Blotting (WB) image for Goat anti-Mouse (Murine) IgG (Heavy & Light Chain) Antibody (PE) (ABIN2669887) Dot Blot showing the detection of Mouse IgG. A three-fold serial dilution of Mouse Ig...