Western Blot of Alkaline Phosphatase Conjugated Goat Anti-Mouse IgG Antibody Lane 1: Mouse IgG Load: 100 ng per lane Secondary antibody: Alkaline Phosphatase Conjugated Goat Anti-Mouse IgG Antibody at 1:1000 for 60 min at RT Block: MB-070 for 30 min RT Predicted/Observed size: 55 and 28 kDa, 55 and 28 kDa