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  • 产品名称：Mouseanti-HumanIg(Chainlambda),(LightChain)Antibody(APC)

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  • 产品厂商：BioLeaf
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简单介绍：
Mouseanti-HumanIg(Chainlambda),(LightChain)Antibody(APC)
详情介绍：
Clone 4C2
Isotype IgG1
Specificity The antibody 4C2 reacts with lambda light chains (22.5 kDa) of human immunoglobulin.
No Cross-Reactivity Hamster, Sheep (Ovine), Goat, Rabbit, Guinea Pig
Characteristics The purified antibody is conjugated with cross-linked Allophycocyanin (APC) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use. No reconstitution is necessary.
Background Immunoglobulin classes share the same basic four polypeptide chain structure of two heavy chains (five heavy chains types) and two light chains (kappa, lambda, both having a molecular weight of 22.5 kDa). Kappa and lambda consist of a variable region and a constant region and can easily be differentiated by the antigenic properties of the constant region. The ratio of kappa to lambda is 70:30.
Molecular Weight 22.5 kDa
Application Notes The reagent is designed for Flow Cytometry analysis of human blood cells using 10 μ,l reagent / 100 μ,l of whole blood or 106 cells in a suspension.The content of a vial (1 ml) is sufficient for 100 tests.
Restrictions For Research Use only
Reconstitution No reconstitution is necessary.
Buffer The reagent is provided in phosphate buffered saline (PBS) containing 15 mM sodium azide and 0.2 % (w/v) high-grade protease free Bovine Serum Albumin (BSA) as a stabilizing agent.
Handling Advice Do not freeze.
Avoid prolonged exposure to light.
Storage 4 °C
Storage Comment Store in the dark at 2-8°C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.
Supplier Images
Flow Cytometry (FACS) image for Mouse anti-Human Ig (Chain lambda), (Light Chain) Antibody (APC) (ABIN343719) B lymphocytes (CD19 +) in a dot-plot Lambda PE vs. Kappa FITC. Kappa light chain dete...
Background publications Franklin: "Structure and function of immunoglobulins." in: Acta endocrinologica. Supplementum, Vol. 194, pp. 77-95, 1975 (PubMed).