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  • 产品名称:Mouseanti-HumanIg(Chainkappa),(LightChain)Antibody

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简单介绍:
Mouseanti-HumanIg(Chainkappa),(LightChain)Antibody
详情介绍:
Immunogen Crude thymus membrane fraction.
Clone MEM-09
Isotype IgG1
Specificity The antibody MEM-09 reacts with both secreted and B cell-surface human immunoglobulin, specifically reacts with kappa light chains (22.5 kDa).
Material immunoprecipitated from human serum with the antibody MEM-09 consists of IgG and traces of IgM.
Purification Purified from ascites by protein-A affinity chromatography.
Purity > 95 % (by SDS-PAGE)
Background Immunoglobulin classes share the same basic four polypeptide chain structure of two heavy chains (five heavy chains types) and two light chains (kappa, lambda, both having a molecular weight of 22.5 kDa). Kappa and lambda consist of a variable region and a constant region and can easily be differentiated by the antigenic properties of the constant region. The ratio of kappa to lambda is 70:30.
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Concentration 1 mg/mL
Buffer Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
Handling Advice Do not freeze.
Storage 4 °C
Storage Comment Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label.
Supplier Images
Western Blotting (WB) image for Mouse anti-Human Ig (Chain kappa), (Light Chain) Antibody (ABIN94406) Western Blotting analysis (reducing conditions) of human blood sera using anti-human ...
Background publications Franklin: "Structure and function of immunoglobulins." in: Acta endocrinologica. Supplementum, Vol. 194, pp. 77-95, 1975 (PubMed).