产品详情
所在位置: 首页> 产品目录> 二抗>
查看大图

  • 产品名称：Donkeyanti-GoatIgG(Heavy&LightChain)Antibody(HRP)

  • 产品型号：
  • 产品厂商：BioLeaf
  • 产品文档：
你添加了1件商品 查看购物车
简单介绍：
Donkeyanti-GoatIgG(Heavy&LightChain)Antibody(HRP)
详情介绍：
Immunogen Full-length goat IgG (purified)
Isotype IgG
Characteristics Donkey Anti-Goat IgG (H&L) [HRP] Polyclonal Antibody is highly purified from donkey antiserum by immunoaffinity chromatography and then conjugated to horseradish peroxidase. It reacts with goat IgG heavy and light chains.
Purification Immunoaffinity chromatography
Research Area Immunology, Secondary Antibodies
Application Notes Donkey Anti-Goat IgG (H&L) [HRP] is suitable for immunoblotting (western and dot blots), ELISA, immunoperoxidase electron microscopy, immunohistochemistry, and other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.The investigator must determine the ideal working concentration for each specific application. The ideal working concentration must take into account such factors as secondary antibody affinity, antigen concentration, sensitivity of the detection method, temperature, and the length of the incubations. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.ELISA: 1:10,000-1:100,000
Western blot: 1:1,000-1:20,000Other applications: user-optimized
Restrictions For Research Use only
Format Lyophilized
Reconstitution Reconstitute the lyophilized powder with 1 mL of deionized water (or equivalent) to antibody concentration of 1 mg/mL.
Concentration 1 mg/mL
Buffer PBS, pH 7.4, containing 1 % BSA and 0.01 % thimerosal.
Preservative Thimerosal (Merthiolate)
Precaution of Use Biohazard Informations: This product contains thimerosal which is hazardous.
Handling Advice Avoid repeated freezing and thawing. Dilute only prior to immediate use
Centrifuge product if not completely clear after standing at room temperature.
Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.
Storage -20 °C
Storage Comment The antibody is stable in lyophilized form if stored at -20 °C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-0 °C. For long term storage, aliquot and store at -20 °C or below.
Product cited in: Liu, Jiang, Xing, Huang, Mao, He: "Uncoupling protein 2 expression affects androgen synthesis in polycystic ovary syndrome." in: Endocrine, Vol. 43, Issue 3, pp. 714-23, 2013 (PubMed).

Stengel, Goebel-Stengel, Wang, Shaikh, Lambrecht, Rivier, Taché: "Abdominal surgery inhibits circulating acyl ghrelin and ghrelin-O-acyltransferase levels in rats: role of the somatostatin receptor subtype 2." in: American journal of physiology. Gastrointestinal and liver physiology, Vol. 301, Issue 2, pp. G239-48, 2011 (PubMed).

Stengel, Goebel, Wang, Reeve, Taché, Lambrecht: "Lipopolysaccharide differentially decreases plasma acyl and desacyl ghrelin levels in rats: potential role of the circulating ghrelin-acylating enzyme GOAT." in: Peptides, Vol. 31, Issue 9, pp. 1689-96, 2010 (PubMed).