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  • 产品名称：NEDD4293CellLysate

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  • 产品厂商：Abbiotec
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NEDD4293CellLysate
详情介绍：
Characteristics Antigen standard for neural precursor cell expressed, developmentally down-regulated 4 (NEDD4), transcript variant 1 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid (OriGene Technologies, Inc.) and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Components This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer.
Alternative Name NEDD4
Background Synonyms: isoform 1 is encoded by transcript variant 1, receptor-potentiating factor 1, NEDD-4, cell proliferation-inducing gene 53 protein, neural precursor cell expressed developmentally down-regulated protein 4, E3 ubiquitin-protein ligase NEDD4, NEDD4, KIAA0093, MGC176705, NEDD4-1, RPF1
NCBI Accession NP_006145
Research Area Chromatin and Nuclear Signaling, Cell Cycle, Proteolysis / Ubiquitin
Application Notes WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 µg lysate per lane.
Restrictions For Research Use only
Format Liquid
Buffer Each lysate vial contains 0.1 mg lysate in 0.1 mL (1 mg/mL) of RIPA Buffer (50 mM Tris-HCl pH 7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1 % NP40). The loading buffer vial contains 0.5 mL 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10 % glycerol, 4 % SDS, 0.002 % Bromophenol blue, 5 % beta-mercaptoethanol).
Handling Advice Minimize freeze-thaw cycles.
Storage -80 °C
Storage Comment Store at -80 °C. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20 °C.
Expiry Date 6 months