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  • 产品名称：MARCH8293CellLysate

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  • 产品厂商：Abbiotec
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MARCH8293CellLysate
详情介绍：
Characteristics Antigen standard for membrane-associated ring finger (C3HC4) 8 (MARCH8), transcript variant 6 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid (OriGene Technologies, Inc.) and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Components This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer.
Alternative Name MARCH8
Background Synonyms: isoform 6 is encoded by transcript variant 6, c-mir, cellular modulator of immune recognition, membrane-associated RING-CH protein VIII, cellular modulator of immune recognition (c-MIR), RING finger protein 178, membrane-associated RING finger protein 8, 40245, c-MIR, MARCH-VIII, MIR, RNF178
NCBI Accession NP_001002265
Research Area Proteolysis / Ubiquitin, Adaptive Immunity
Application Notes WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 µg lysate per lane.
Restrictions For Research Use only
Format Liquid
Buffer Each lysate vial contains 0.1 mg lysate in 0.1 mL (1 mg/mL) of RIPA Buffer (50 mM Tris-HCl pH 7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1 % NP40). The loading buffer vial contains 0.5 mL 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10 % glycerol, 4 % SDS, 0.002 % Bromophenol blue, 5 % beta-mercaptoethanol).
Handling Advice Minimize freeze-thaw cycles.
Storage -80 °C
Storage Comment Store at -80 °C. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20 °C.
Expiry Date 6 months