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  • 产品名称：FibroblastGrowthFactor2(Basic)(FGF2)(AA143-288)Peptide

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  • 产品厂商：BioLeaf
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简单介绍：
FibroblastGrowthFactor2(Basic)(FGF2)(AA143-288)Peptide
详情介绍：
Sequence MPALPEDGGS GAFPPGHFKD PKRLYCKNGG FFLRIHPDGR VDGVREKSDP HIKLQLQAEE RGVVSIKGVC ANRYLAMKED GRLLASKCVT DECFFFERLE SNNYNTYRSR KYTSWYVALK RTGQYKLGSK TGPGQKAILF LPMSAKS
Characteristics Purified recombinant Human FGF2 protein
Expression System: E.coli
Bioactivity: The ED50 for this effect is 0.1-1.5 ng/mL. Measured in a cell proliferation assay using NIH-3T3 mouse embryonic fibroblast.
Molecular weight on SDS-PAGE will appear higher.
Purity > 95 % pure
Endotoxin Level < 1.0 EU per µg of protein (determined by LAL method)
Background Fibroblast growth factor 2 (FGF2) is a member of the FGF family that binds heparin and plays important roles in morphogenic, neurotrophic and angiogenic processes. FGF2 possess diverse biological functions, such as neuron differentiation, embryonic development and differentiation, modulation of angiogenesis, and wound healing. Recombinant human FGF2 was expressed in E. coli and purified by conventional chromatography techniques.
Alternative Names: FGF-2, FGF 2, BFGF protein, fibroblast growth factor 2 protein, FGF2, FGF-2 protein, FGFB protein, FGF 2 protein, HBGF-2 protein, FGF2 protein, fibroblast growth factor 2
Molecular Weight 16.5 kDa (147 AA)
Research Area Cytokines, Growth Factors
Application Notes FGF2 protein has been used in SDS PAGE and may be suitable for use in other assays to be determined by the end user.
Assay Procedure
  1. Cell line: NIH-3T3 (Mus mosculus/Mouse embryonic fibroblast )
    2. Maintenance Condition: RPMI 1640 containing 10 % FBS
    3. Assay medium: serum free RPMI 1640
    4. Cell density: 2 x 10,000 cells/well (96 well plate, final volume 100 µL)
    5. Incubation time : 24 hr (after sample treatment)
    6. Concentration range: 10 pg/mL - 1 µg/mL 7. Detection method : BrdU assay
Restrictions For Research Use only
Format Liquid
Concentration 1 mg/mL
Buffer Supplied as a liquid in 50 mM Tris, pH 8.0.
Handling Advice Avoid repeated freeze/thaw cycles.
Storage RT/-20 °C
Storage Comment Store at 4 °C for short term storage (1/2 weeks). Aliquot and store at -20 °C or - 70 °C for long term storage.
Supplier Images
SDS-PAGE (SDS) image for Fibroblast Growth Factor 2 (Basic) (FGF2) (AA 143-288) Peptide (ABIN934996) Figure annotation denotes ug of protein loaded and % gel used.
Product cited in: Borello, Cobos, Long, McWhirter, Murre, Rubenstein: "FGF15 promotes neurogenesis and opposes FGF8 function during neocortical development." in: Neural development, Vol. 3, pp. 17, 2008 (PubMed).