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  • 产品名称:Goatanti-Dog(Canine)IgG(Heavy&LightChain)Antibody

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  • 产品厂商:Agrisera
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简单介绍:
Goatanti-Dog(Canine)IgG(Heavy&LightChain)Antibody
详情介绍:
Immunogen purified dog normal IgG isolated from pooled dog serum
Cross-Reactivity (Details) Not reactive in: This antibody does not react with any non-Ig protein in dog serum, as tested by immunoelectrophoresis and double radial immunodiffusion.
Characteristics Goat anti-Dog IgG (H&L), is a secondary antibody which binds to dog IgG heavy and light chain in immunological assays.
Research Area Immunology, Secondary Antibodies
Application Notes Immunoprecipitation. In immunoelectrophoresis use 2 mL or equivalent against 120 mL antiserum. In double radial immunodiffusion (Ouchterlony) use a rosette arrangement with 10 mL antiserum in a 3 mm diameter centre well and 2 mL serum samples (neat and diluted) in 2 mm diameter peripheral wells. Precipitating titre not less than 1:256 when tested against normal dog serum in agar block titration.
Comment

Inter-species cross-reactivity is a normal feature of antibodies to immunoglobulins, since Ig of different species frequently share antigenic determinants. Cross-reactivity of this antiSerum has not been tested in detail. No preservative added.The reactivity of the antiserum is directed to the Fc and Fab subunits of the IgG molecule. It includes a certain degree of reactivity with other immunoglobulins via the common Fab portion.

Restrictions For Research Use only
Format Lyophilized
Reconstitution For reconstitution please add 1 mL of sterile destilled water.
Storage -20 °C
Storage Comment store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes. Reconstituted material can be stoored in +4°C up to 7 days.