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  • 产品名称：Goatanti-RabbitIgG(Heavy&LightChain)Antibody(DyLight550)

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  • 产品厂商：Agrisera
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简单介绍：
Goatanti-RabbitIgG(Heavy&LightChain)Antibody(DyLight550)
详情介绍：
Cross-Reactivity Sheep (Ovine), Goat, Human, Mouse (Murine), Rat (Rattus)
Characteristics Goat anti-rabbit IgG (H&L), DyLight® 550 conjugated, is a secondary antibody which binds to rabbit IgGs in immunological assays. DyLight® 550 has Amax = 562 nm, Emax = 576 nm. Fluor to protein ratio is 2. 47 moles DyLight® 550 per mole antibody. Antibodies are adsorbed against bovine, goat, human, mouse, rat IgG and are affinity purified using solid phase rabbit IgG.
Goat anti-rabbit IgG (H&L), DyLight 550 conjugated, is a secondary antibody which binds to rabbit IgGs in immunological assays. DyLight 550 has Amax = 562 nm, Emax = 576 nm. Fluor to protein ratio is 2.47 moles DyLight 550 per mole antibody. Antibodies are adsorbed against bovine,goat,human,mouse,rat IgG and are affinity purified using solid phase rabbit IgG.
Purification Affinity purification
Research Area Immunology, Secondary Antibodies
Application Notes 1:20-1: 2000 for immunohistochemistry (IHC), 1:50-1: 5000 for immunocytochemistry (ICC).,1:20-1: 2000 for immunohistochemistry (IHC), 1:50-1: 5000 for immunocytochemistry (ICC).,1:20-1: 2000 for immunohistochemistry (IHC), 1:50-1: 5000 for immunocytochemistry (ICC).,1:20-1: 2000 for immunohistochemistry (IHC), 1:50-1: 5000 for immunocytochemistry (ICC).
Restrictions For Research Use only
Format Lyophilized
Reconstitution For reconstitution add 1.1 mL of sterile water. Let it stand 30 minutes at room temperature to dissolve. Prepare fresh working dilutions daily.
Handling Advice Product is photosensitive and should be protected from light.
Storage 4 °C/-20 °C
Storage Comment Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°C after rehydration.For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1.1 ml of sterile water add 1.1 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.,Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°C after rehydration.For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1.1 ml of sterile water add 1.1 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.