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  • 产品名称：Donkeyanti-Mouse(Murine)IgG(Heavy&LightChain)Antibody(DyLight650)

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  • 产品厂商：Agrisera
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简单介绍：
Donkeyanti-Mouse(Murine)IgG(Heavy&LightChain)Antibody(DyLight650)
详情介绍：
Immunogen Purified mouse IgG
Specificity Based on immunoelectrophoresis, this antibody reacts with: heavy (γ) chains on mouse IgG and light chains on all mouse immunoglobulins.
Characteristics Donkey anti-Mouse IgG (H&L) - Affinity Pure, DyLight650 Conjugate, is a secondary antibody conjugated to DyLight 594, which binds to all anti-mouse IgG in immunological assays. Amax = 593 nm, Emax = 618 nm.Antibodies are are affinity purified using solid phase mouse IgG.
Purification Affinity purification
Research Area Immunology, Secondary Antibodies
Application Notes 1:20 - 1:2 000 for most applications
Comment

immunolocalization Donkey anti-mouse conjugated to DY Light 650 in the absence of primary antibody show very little background label (left images) and binds to primary antibody (anti-i±-tubulin, right images). The top images show only the antibody label in red and bottom images show overlay of antibody label in red with DAPI in blue and DIC/Nomarski image in grey. The material has been prepared using whole mount immunolabelling procedure of 5 day old Arabidopsis thaliana seedlings (Fischer, U. et al. (2006). Curr. Biol. 16, 2143-2149). Mouse anti-i±-tubulin DM1A (Santa Cruz, Sc-32293) was diluted 1:200 and Donkey anti-mouse DY Light 650 was diluted 1:100. DNA was stained with DAPI. Courtesy: Dr. Anna Gustavsson and Prof. Markus Grebe, UmeA Plant Science Centre
Restrictions For Research Use only
Format Lyophilized
Reconstitution For reconstitution add 1.1 mL of sterile water. Let it stand 30 minutes at room temperature to dissolve. Prepare fresh working dilutions daily.
Handling Advice Product is photosensitive and should be protected from light.
Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
A solution with 50 % glycerol will not freeze in -20 °C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol.
Storage 4 °C/-20 °C
Storage Comment Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°C after rehydration.For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1.1 ml of sterile water add 1.1 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.