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  • 产品名称：Goatanti-HumanIgE(Chainepsilon)Antibody(AlkalinePhosphatase(AP))

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  • 产品厂商：Agrisera
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简单介绍：
Goatanti-HumanIgE(Chainepsilon)Antibody(AlkalinePhosphatase(AP))
详情介绍：
Immunogen purified human IgE
Isotype IgG
Cross-Reactivity Sheep (Ovine), Mouse (Murine), Rabbit
Predicted Reactivity human IgE heavy (epsilon chain)
Characteristics Goat anti-human IgE heavy (epsilon chain) is a secondary antibody conjugated to AP (Alkaline phosphatase) which binds to human IgE heavy (epsilon chain) in immunological assays.
Purification Affinity purification
Research Area Immunology, Secondary Antibodies
Application Notes 1: 500 - 2 000 (ELISA) and (WB), 1: 20 -1: 2000 (IHC), 1: 50 - 1: 5000 (ICC)
Comment

No reactivity is observed to light chains or non-IgE human serum proteins based in immunoelectrophoresisno reactivity to bovine, mouse or rabbit serum proteins based on immunoelectrophoresis
Restrictions For Research Use only
Format Liquid
Handling Advice Do not freeze! Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
Do not add Sodium azide.
Each reagent is stable for the period shown on the bottle label if stored as directed.
Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming
A solution with 50 % glycerol will not freeze in -20 °C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol.
Storage 4 °C
Storage Comment Non-diluted antibody is stable for 4 years at 2-8°C. For storage at -20°C dilute antibody solution with an equal volume of glycerol to obtain final glycerol concentration of 50 % to prevent loss of enzymatic activity. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.