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  • 产品名称：ATPase,H+Transporting,Lysosomal38kDa,V0SubunitD2(ATP6V0D2)Peptide

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  • 产品厂商：Agrisera
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简单介绍：
ATPase,H+Transporting,Lysosomal38kDa,V0SubunitD2(ATP6V0D2)Peptide
详情介绍：
Blocked Antibody anti-ATP6V0D2 antibody (ATPase, H+ Transporting, Lysosomal 38kDa, V0 Subunit D2) (Isoform 1) (ABIN3197488)
Specificity KLH-conjugated synthetic peptide chosen from subunit E of plant V-ATPase including Arabidopsis thaliana At4g11150. Peptide is conserved in vacuolar H+-ATPase subunit E, isoform 1, isoform 2, isoform 3 (VHA-E1).
Characteristics Plant vacuole V-ATPase is responsible for energization of transport of ions and metabolites, and acts as well 'house-keeping' and as a stress response enzyme. V-ATPase is a multi-subunit enzyme composed of a membrane sector and a cytosolic catalytic sector. It is related to the FoF1 ATP synthase. Alternative protein names: Vacuolar proton pump subunit E, Protein EMBRYO DEFECTIVE 2448
Purification affinity purified
Background This blocking peptide can be used as a control to neutralize V-ATPase | epsilon subunit of tonoplast H+ATPase before immunolocalization or western blot. Furter details are provided below.Plant vacuole V-ATPase is responsible for energization of transport of ions and metabolites, and acts as well 'house-keeping' and as a stress response enzyme. V-ATPase is a multi-subunit enzyme composed of a membrane sector and a cytosolic catalytic sector. It is related to the FoF1 ATP synthase. Alternative protein names: Vacuolar proton pump subunit E, Protein EMBRYO DEFECTIVE 2448
Molecular Weight 3049.5 g/mol
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Format Liquid
Concentration 2 μg/μL
Handling Advice Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from the material adhering to the cap or sides of the tubes.
Make aliquotses to avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment store at -20°C, make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from liquid material adhering to the cap or sides of the tubes.
Background publications Bohne, Schwarz, Schottkowski, Lidschreiber, Piotrowski, Zerges, Nickelsen: "Reciprocal regulation of protein synthesis and carbon metabolism for thylakoid membrane biogenesis." in: PLoS biology, Vol. 11, Issue 2, pp. e1001482, 2013 (PubMed).

Pertl, Schulze, Obermeyer: "The pollen organelle membrane proteome reveals highly spatial-temporal dynamics during germination and tube growth of lily pollen." in: Journal of proteome research, Vol. 8, Issue 11, pp. 5142-52, 2009 (PubMed).

Product cited in: Lang, Mueller, Hoernstein, Porankiewicz-Asplund, Vervliet-Scheebaum, Reski: "Simultaneous isolation of pure and intact chloroplasts and mitochondria from moss as the basis for sub-cellular proteomics." in: Plant cell reports, Vol. 30, Issue 2, pp. 205-15, 2011 (PubMed).

Hinton, Sennoune, Bond, Fang, Reuveni, Sahagian, Jay, Martinez-Zaguilan, Forgac: "Function of a subunit isoforms of the V-ATPase in pH homeostasis and in vitro invasion of MDA-MB231 human breast cancer cells." in: The Journal of biological chemistry, Vol. 284, Issue 24, pp. 16400-8, 2009 (PubMed).