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  • 产品名称：THP-1nuclearextract(TPAstimulated)

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  • 产品厂商：ActiveMotif
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简单介绍：
THP-1nuclearextract(TPAstimulated)
详情介绍：
Characteristics

Origin: human monocytes
Components 2 x 100 μg of THP-1 nuclear extract (PMA stimulated) at 2.50 μg/μl
Restrictions For Research Use only
Concentration 2.50 μg/μL
Buffer The THP-1 nuclear extracts have been collected in Lysis Buffer after a 24-hour incubation with PMA (10 ng/mL). The Lysis Buffer consists of 20 mM Hepes pH 7.5, 400 mM NaCl, 20 % glycerol, 0.1 mM EDTA, 10 mM NaF, 10 μM Na2MoO4, 1 mM NaVO3, 10 mM PNPP, 10 mM b-glycerophosphate, 1 mM DTT and protease inhibitors. The protein content has been determined by a Bradford-based assay.
Preservative Dithiothreitol (DTT)
Precaution of Use This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice It ist recommended to aliquot the extracts into single-use fractions and then storing them at -80 °C. This eliminates repeated freeze/thaw cycles.
Storage -80 °C
Storage Comment To ensure stability, extracts should be stored at -80°C.