Buffer
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Nuclear extracts are supplied in dilution buffer (20 mM Hepes ( pH 7.9), 100 mM KCl, 1 mM MgCl2, 20 % glycerol, 0.5 mM PMSF and 0.5 mM DTT). Cells were cultured in medium supplemented with 5 nM TPA (phorbol, 12-myristate, 13 acetate) for 72 hours at 37 °C immediately prior to harvesting.
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