Buffer
|
The HeLa whole-cell extracts have been collected in Lysis Buffer after a 30-minute incubation with or without TNF-α (20 ng/mL). The Lysis Buffer consists of 20 mM Hepes pH 7.5, 350 mM NaCl, 20 % glycerol, 1 % Igepal-CA630, 1 mM MgCl2, 0.5 mM EDTA and 0.1 mM EGTA. The protein content has been determined by a Bradford-based assay.
|