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  • 产品名称：HistoneCluster3,H3(HIST3H3)(pThr3),(N-Term),(truncated),(fulllength)Protein

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  • 产品厂商：ActiveMotif
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简单介绍：
HistoneCluster3,H3(HIST3H3)(pThr3),(N-Term),(truncated),(fulllength)Protein
详情介绍：
Characteristics Recombinant Histone H3 phospho Thr3 (H3Thr3ph) proteins are generated using expressed protein ligation (EPL) technology. Truncated human Histone H3.2 is produced in E. coli and purified using FPLC. The purified protein is subsequently ligated to a N-terminal histone tail peptide containing phosphorylated threonine 3 via a native peptide bond. The full-length protein is then repurified prior to lyophilization. Protein concentration was determined using the molar extinction coefficient for Histone H3 and absorbance at 280nm. The recombinant histone is >98 % pure by SDS-PAGE and confirmed by high-resolution ESI-TOF mass spectrometry. The molecular weight of the recombinant histone is 15,306 Daltons.
Purification Purified using FPLC
Purity The recombinant histone is >98 % pure by SDS-PAGE and confirmed by high-resolution ESI-TOF mass spectrometry.
Background Histone H3 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 146 base pairs of DNA wrapped around an octamer of core histone proteins (two each of H2A, H2B, H3 and H4). Histone H1 is a linker protein, present at the interface between the nucleosome core and DNA entry/exit points.
Molecular Weight The molecular weight of the recombinant histone is 15,306 Daltons.
Research Area Chromatin and Nuclear Signaling, DNA/RNA, Transcription Factors
Application Notes Recombinant histones are suitable for use as positive controls in the analysis of histone post-translational modifications, as substrates for histone modification enzymes, or to generate chromatin in vitro.
Restrictions For Research Use only
Format Lyophilized
Reconstitution Recombinant histones can be resuspended in water or any suitable buffer. We recommend a starting concentration of 1 mg/mL. To fully solubilize the histone we suggest resuspension in the buffer of choice at room temperature for 20-30 minutes with occasional pipetting. Addition of salt or Tris to the resuspension buffer may enhance histone solubility.
Handling Advice Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
Storage -20 °C/-80 °C
Storage Comment Lyophilized proteins can be stored at -20°C or -80°C, preferably desiccated. Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation.
Supplier Images
Western Blotting (WB) image for Histone Cluster 3, H3 (HIST3H3) (pThr3), (N-Term), (truncated), (full length) Protein (ABIN2669562) Recombinant Histone H3 phospho Thr3 tested by SDS-PAGE gel. SDS-PAGE analysis of 0.5 ...
Mass Spectrometry (MS) image for Histone Cluster 3, H3 (HIST3H3) (pThr3), (N-Term), (truncated), (full length) Protein (ABIN2669562) Histone Cluster 3, H3 (HIST3H3) (pThr3), (N-Term), (truncated), (full length) protein (Image 2)
Mass Spectrometry (MS) image for Histone Cluster 3, H3 (HIST3H3) (pThr3), (N-Term), (truncated), (full length) Protein (ABIN2669562) Histone Cluster 3, H3 (HIST3H3) (pThr3), (N-Term), (truncated), (full length) protein (Image 3)