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  • 产品名称：CathepsinL1(CTSL1)(Active)protein(Histag,HeavyLabeled)

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  • 产品厂商：ACROBiosystems
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简单介绍：
CathepsinL1(CTSL1)(Active)protein(Histag,HeavyLabeled)
详情介绍：
Characteristics This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 36.7 kDa. The protein migrates as 10 kDa and 34 kDa on a SDS-PAGE gel under reducing (R) condition due to glycosylation,corresponding to the propeptide and the mature form respectively..
Purity >98 % as determined by SDS-PAGE.
Endotoxin Level Less than 1.0 EU per μg by the LAL method.
Background Cathepsin L (CTSL1) is also known as major excreted protein (MEP), is a member of the peptidase C1 family, is a dimer composed of disulfide-linked heavy and light chains linked by disulfide bonds. CTSL1 is a lysosomal cysteine proteinase that plays a major role in intracellular protein catabolism. Its substrates include collagen and elastin, as well as alpha-1 protease inhibitor, a major controlling element of neutrophil elastase activity. MEP has been implicated in several pathologic processes, including myofibril necrosis in myopathies and in myocardial ischemia, and in the renal tubular response to proteinuria. CTSL1 is important for the overall degradation of proteins in lysosomes. The specificity of MEP is close to that of papain. As compared to cathepsin B, cathepsin L exhibits higher activity toward protein substrates, but has little activity on Z - Arg - Arg - NHMec, and no peptidyl - dipeptidase activity. Human Cathepsin L activity is greatest under mildly acidic conditions, from pH 4.5 - 6.5. The stability of the enzyme decreases at higher pH values
Molecular Weight 36.7 kDa
NCBI Accession NP_001903
UniProt P07711
Research Area Signaling, Extracellular Matrix, Cancer, Proteases
Pathways Activation of Innate immune Response, Toll-Like Receptors Cascades
Restrictions For Research Use only
Format Lyophilized
Reconstitution For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Buffer 50 mM Sodium Acetate, pH 5.0 with 100 mM NaCl
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment Lyophilized Protein should be stored at -20 °C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20 °C or -70 °C. Avoid repeated freeze-thaw cycles.