产品详情
查看大图

  • 产品名称：FcFragmentofIgG,Receptor,Transporter,alpha(FCGRT)(Active)protein(StrepTag,AVIta

  • 产品型号：
  • 产品厂商：ACROBiosystems
  • 产品文档：
你添加了1件商品 查看购物车
简单介绍：
FcFragmentofIgG,Receptor,Transporter,alpha(FCGRT)(Active)protein(StrepTag,AVItag,Biotin,Histag)
详情介绍：
Brand MABSol®,PrecisionAvi
Specificity Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
Characteristics The subunit FCGRT carries an Avi tag (Avitag™) at the C-terminus, followed by a polyhistidine tag. The subunit Beta-2 microglobulin (B2M) carries a Twin Strep tag at the C-terminus. The protein has a calculated MW of 34.7 kDa (FCGRT), 14.7 kDa (B2M) . The protein migrates as 45-50 kDa (FCGRT), 15 kDa (B2M) respectively on a SDS-PAGE gel under reducing (R) condition due to glycosylation.
Purity >95 % as determined by SDS-PAGE.
Endotoxin Level Less than 1.0 EU per μg by the LAL method.
Background FCGRT & B2M heterodimer protein (FcRn complex) consist of two subunits: p51 (equivalent to FCGRT), and p14 (equivalent to beta-2-microglobulin), and forms an MHC class I-like heterodimer. Fc fragment of IgG, receptor, transporter, alpha (FCGRT) binds to the Fc region of monomeric immunoglobulins gamma and mediates the uptake of IgG from milk. FCGRT possible role in transfer of immunoglobulin G from mother to fetus. Beta-2-microglobulin (B2M) is a component of the class I major histocompatibility complex (MHC) and involved in the presentation of peptide antigens to the immune system.
Molecular Weight 34.7 kDa (FCGRT), 14.7 kDa (B2M)
Pathways Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process
Comment

Ready-to-use AvitagTM biotinylated protein:
The product is exclusively produced using the AvitagTM technology. Briefly, a unique 15 amino acid peptide, the Avi tag, is introduced into the recombinant protein during expression vector construction. The single lysine residue in the Avi tag is enzymatically biotinylated by the E. Coli biotin ligase BirA.

This single-point enzymatic labeling technique brings many advantages for commonly used binding assays. The biotinylation happens on the lysine residue of Avi tag, and therefore does NOT interfere with the target protein's natural binding activities. In addition, when immobilized on an avidin-coated surface, the protein orientation is uniform because the position of the Avi tag in the protein is precisely controlled.
Restrictions For Research Use only
Format Lyophilized
Reconstitution Please see Certificate of Analysis for specific instructions. For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Buffer PBS, pH 7.4
Storage -20 °C
Supplier Images
SDS-PAGE (SDS) image for Fc Fragment of IgG, Receptor, Transporter, alpha (FCGRT) (Active) protein (Strep Tag,AVI tag,Biotin,His tag) (ABIN2870570) Biotinylated Mouse FcRn / FCGRT & B2M on SDS-PAGE under reducing (R) condition. The g...
Binding Studies (Bind) image for Fc Fragment of IgG, Receptor, Transporter, alpha (FCGRT) (Active) protein (Strep Tag,AVI tag,Biotin,His tag) (ABIN2870570) Immobilized Yervoy Ipilimumab (Human IgG1) at 10 μg/mL (100 μL/well) can bind Biotiny...