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  • 产品名称：Ubiquitin-ConjugatingEnzymeE2H(UBE2H)(Active)protein(GSTtag)

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  • 产品厂商：ACROBiosystems
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简单介绍：
Ubiquitin-ConjugatingEnzymeE2H(UBE2H)(Active)protein(GSTtag)
详情介绍：
Characteristics This protein carries a GST tag at the N-terminus. The protein has a calculated MW of 47.6 kDa. The protein migrates as 45 kDa under reducing (R) condition (SDS-PAGE).
Purity >95 % as determined by SDS-PAGE.
Endotoxin Level Less than 1.0 EU per μg by the LAL method.
Background Ubiquitin-conjugating enzyme E2 H (UBE2H), a member of the ubiquitin-conjugating enzyme family, is also known as ubiquitin carrier protein H, ubiquitin-conjugating enzyme E2-20K and ubiquitin-protein ligase H. The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. At the same time, ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. Furthermore,UBE2H can accept ubiquitin from the E1 complex and catalyze its covalent attachment to other proteins. In vitro,UBE2H can aslo catalyze 'Lys-11'- and 'Lys-48'-linked polyubiquitination, and it is capable, in vitro, to ubiquitinate histone H2A.
Molecular Weight 47.6 kDa
Restrictions For Research Use only
Format Lyophilized
Reconstitution Please see Certificate of Analysis for specific instructions. For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Buffer 50 mM HEPES, 150 mM NaCl, pH 7.5
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment Lyophilized Protein should be stored at -20 °C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20 °C or -70 °C. Avoid repeated freeze-thaw cycles.
Supplier Images
SDS-PAGE (SDS) image for Ubiquitin-Conjugating Enzyme E2H (UBE2H) (Active) protein (GST tag) (ABIN2444230) Human UbcH2, GST Tag on SDS-PAGE under reducing (R) condition. The gel was stained ov...