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  • 产品名称：ProgrammedCellDeath1Ligand2(PDCD1LG2)(Active)protein(FcTag,AVItag,Biotin)

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  • 产品厂商：ACROBiosystems
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简单介绍：
ProgrammedCellDeath1Ligand2(PDCD1LG2)(Active)protein(FcTag,AVItag,Biotin)
详情介绍：
Brand MABSol®,PrecisionAvi
Specificity Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
Characteristics This protein carries a human IgG1 Fc fragment at the C-terminus, followed by an Avi tag (Avitag™). The protein has a calculated MW of 51 kDa. The protein migrates as 50-55 kDa and 60-70 kDa on a SDS-PAGE gel under reducing (R) condition due to glycosylation.
Purity >95 % as determined by reduced SDS-PAGE.
Endotoxin Level Less than 1.0 EU per μg by the LAL method.
Background Programmed cell death 1 ligand 2 (PD-L2 or PDCD1 ligand 2) is also known as Butyrophilin B7-DC, CD antigen CD273, which belongs to the immunoglobulin superfamily or BTN/MOG family. The expression of PD-L2 is up-regulated by IFNG/IFN-gamma stimulation in monocytes and induced on dendritic cells grown from peripheral blood mononuclear cells with CSF2 and IL-4. PD-L2 Involved in the costimulatory signal, essential for T-cell proliferation and IFNG production in a PDCD1-independent manner. PD-L2 interaction with PDCD1 inhibits T-cell proliferation by blocking cell cycle progression and cytokine production.
Molecular Weight 51 kDa
UniProt Q9BQ51
Comment

Ready-to-use AvitagTM biotinylated protein:
The product is exclusively produced using the AvitagTM technology. Briefly, a unique 15 amino acid peptide, the Avi tag, is introduced into the recombinant protein during expression vector construction. The single lysine residue in the Avi tag is enzymatically biotinylated by the E. Coli biotin ligase BirA.

This single-point enzymatic labeling technique brings many advantages for commonly used binding assays. The biotinylation happens on the lysine residue of Avi tag, and therefore does NOT interfere with the target protein's natural binding activities. In addition, when immobilized on an avidin-coated surface, the protein orientation is uniform because the position of the Avi tag in the protein is precisely controlled.
Restrictions For Research Use only
Format Lyophilized
Reconstitution For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Buffer 50 mM tris, 100 mM glycine, pH 7.5
Storage -20 °C
Supplier Images
SDS-PAGE (SDS) image for Programmed Cell Death 1 Ligand 2 (PDCD1LG2) (Active) protein (Fc Tag,AVI tag,Biotin) (ABIN2870562) Biotinylated Human PD-L2, Fc Tag on SDS-PAGE under reducing (R) condition. The gel wa...
Binding Studies (Bind) image for Programmed Cell Death 1 Ligand 2 (PDCD1LG2) (Active) protein (Fc Tag,AVI tag,Biotin) (ABIN2870562) Immobilized Human PD-1, Fc Tag (HPLC-verified) (Cat# PD1-H5257) at 2μg/mL (100 µl/wel...