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  • 产品名称：GrowthFactor,AugmenterofLiverRegeneration(GFER)(Active)protein(Histag,SUMOTag,H

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  • 产品厂商：ACROBiosystems
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简单介绍：
GrowthFactor,AugmenterofLiverRegeneration(GFER)(Active)protein(Histag,SUMOTag,HeavyLabeled)
详情介绍：
Characteristics This protein carries a polyhistidine tag at the N-terminus, followed by a SUMO tag. The protein has a calculated MW of 27.3 kDa. The protein migrates as 33 kDa on a SDS-PAGE gel under reducing (R) condition.
Purity >80 % as determined by SDS-PAGE.
Endotoxin Level Less than 1.0 EU per μg by the LAL method.
Background Growth factor, augmenter of liver regeneration (GFER) is also known as FAD-linked sulfhydryl oxidase ALR, which belongs to the Erv1/ALR family of proteins. This family can be found in higher and lower eukaryotes. There are two isoform of GFER: Isoform 1 and isoform 2. Isoform 2 missing 1 - 80 aa. Isoform 1 is mainly located in mitochondrion intermembrane space, while Isoform 1 is secreted to cytoplasm. Isoform 1 of GFER regenerates the redox-active disulfide bonds in CHCHD4/MIA40, a chaperone essential for disulfide bond formation and protein folding in the mitochondrial intermembrane space. The reduced form of CHCHD4/MIA40 forms a transient intermolecular disulfide bridge with GFER/ERV1, resulting in regeneration of the essential disulfide bonds in CHCHD4/MIA40, while GFER/ERV1 becomes re-oxidized by donating electrons to cytochrome c or molecular oxygen. The isoform 2 of GFER may act as an autocrine hepatotrophic growth factor promoting liver regeneration.
Molecular Weight 27.3 kDa
NCBI Accession NP_005253
Restrictions For Research Use only
Format Lyophilized
Reconstitution For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Buffer PBS, pH 7.4
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment Lyophilized Protein should be stored at -20 °C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20 °C or -70 °C. Avoid repeated freeze-thaw cycles.
Supplier Images
SDS-PAGE (SDS) image for Growth Factor, Augmenter of Liver Regeneration (GFER) (Active) protein (His tag,SUMO Tag,Heavy Labeled) (ABIN2444131) Growth Factor, Augmenter of Liver Regeneration (GFER) (Active) protein (His tag,SUMO Tag,Heavy Labeled)