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  • 产品名称：Cadherin1,Type1,E-Cadherin(Epithelial)(CDH1)(Active)protein(FcTag)

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  • 产品厂商：ACROBiosystems
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简单介绍：
Cadherin1,Type1,E-Cadherin(Epithelial)(CDH1)(Active)protein(FcTag)
详情介绍：
Characteristics This protein carries a human IgG1 Fc tag at the C-terminus. The protein has a calculated MW of 92.4 kDa. As a result of different glycosylation, the protein migrates as 110-125 kDa Cadherin, a 48 kDa Propeptide under reducing (R) condition, and 200-250 kDa under non-reducing (NR) condition (SDS-PAGE).
Purity >95 % as determined by SDS-PAGE.
Endotoxin Level Less than 1.0 EU per μg by the LAL method.
Background Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells, cadherins may thus contribute to the sorting of heterogeneous cell types. Cadherin-1 (CDH1) is also known as epithelial cadherin (E-cadherin), CD_antigen (CD324), Uvomorulin (UVO) ECAD and CDHE, CDH1 / CD324 contains 5 cadherin domains. CDH1 / CD324 / ECAD is expressed in non-neural epithelial tissues. CDH1 / E-CAD is involved in mechanisms regulating cell-cell adhesions, mobility and proliferation of epithelial cells and has a potent invasive suppressor role. It is a ligand for integrin alpha-E/beta-7. E-Cad promotes non-amyloidogenic degradation of Abeta precursors and has a strong inhibitory effect on APP C99 and C83 production. Defects in CDH1 / CD324 / ECAD are the cause of hereditary diffuse gastric cancer (HDGC).
Molecular Weight 92.4 kDa
UniProt P12830
Pathways Sensory Perception of Sound, Cell-Cell Junction Organization, Tube Formation
Restrictions For Research Use only
Format Lyophilized
Reconstitution Please see Certificate of Analysis for specific instructions. For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Buffer 50 mM Tris, 100 mM Glycine, pH 7.5
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment Lyophilized Protein should be stored at -20 °C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20 °C or -70 °C. Avoid repeated freeze-thaw cycles.
Supplier Images
SDS-PAGE (SDS) image for Cadherin 1, Type 1, E-Cadherin (Epithelial) (CDH1) (Active) protein (Fc Tag) (ABIN2444102) The purity of rhECAD Fc Chimera was determined by SDS-PAGE of reduced (R) and non-red...
Background publications Shojaei, Wu, Zhong, Yu, Liang, Yao, Blanchard, Bais, Peale, van Bruggen, Ho, Ross, Tan, Carano, Meng, Ferrara: "Bv8 regulates myeloid-cell-dependent tumour angiogenesis." in: Nature, Vol. 450, Issue 7171, pp. 825-31, 2007 (PubMed).

Meng, Han, Zhao, Huang, Si, Wu, Mu: "Induction of the LRP16 gene by estrogen promotes the invasive growth of Ishikawa human endometrial cancer cells through the downregulation of E-cadherin." in: Cell research, Vol. 17, Issue 10, pp. 869-80, 2007 (PubMed).

Agiostratidou, Muros, Shioi, Marambaud, Robakis: "The cytoplasmic sequence of E-cadherin promotes non-amyloidogenic degradation of A beta precursors." in: Journal of neurochemistry, Vol. 96, Issue 4, pp. 1182-8, 2006 (PubMed).

Yoon, Ku, Yang, Kim, Park, Park: "Germline mutations of E-cadherin gene in Korean familial gastric cancer patients." in: Journal of human genetics, Vol. 44, Issue 3, pp. 177-80, 1999 (PubMed).