产品详情
查看大图

  • 产品名称：TumorNecrosisFactorReceptorSuperfamily,Member1A(TNFRSF1A)(Active)protein(AVItag

  • 产品型号：
  • 产品厂商：ACROBiosystems
  • 产品文档：
你添加了1件商品 查看购物车
简单介绍：
TumorNecrosisFactorReceptorSuperfamily,Member1A(TNFRSF1A)(Active)protein(AVItag,Histag,Biotin)
详情介绍：
Brand MABSol®,PrecisionAvi
Specificity Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
Characteristics This protein carries an Avi tag (Avitag™) at the C-terminus, followed by a polyhistidine tag. The protein has a calculated MW of 23.9 kDa. The protein migrates as 35-40 kDa on a SDS-PAGE gel under reducing (R) condition due to different glycosylation.
Purity >95 % as determined by SDS-PAGE.
Endotoxin Level Less than 1.0 EU per μg by the LAL method.
Background Tumor necrosis factor receptor 1 (TNF-R1) is also known as Tumor necrosis factor receptor superfamily member 1A (TNFRSF1A), TNFAR, CD antigen CD120a, which belongs to the tumor necrosis factor receptor superfamily. TNF-R1 contains one death domain and four TNFR-Cys repeats. TNF-R1 is the receptor of TNFSF2 / TNF-alpha and homotrimeric TNFSF1 / lymphotoxin - alpha. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. TNF-R1 contributes to the induction of non - cytocidal TNF effects including anti-viral state and activation of the acid sphingomyelinase. Defects in TNFRSF1A are the cause of familial hibernian fever (FHF).
Molecular Weight 23.9 kDa
NCBI Accession NP_001056
UniProt P19438
Pathways NF-kappaB Signaling, Apoptosis, Caspase Cascade in Apoptosis, Hepatitis C
Comment

Ready-to-use AvitagTM biotinylated protein:
The product is exclusively produced using the AvitagTM technology. Briefly, a unique 15 amino acid peptide, the Avi tag, is introduced into the recombinant protein during expression vector construction. The single lysine residue in the Avi tag is enzymatically biotinylated by the E. Coli biotin ligase BirA.

This single-point enzymatic labeling technique brings many advantages for commonly used binding assays. The biotinylation happens on the lysine residue of Avi tag, and therefore does NOT interfere with the target protein's natural binding activities. In addition, when immobilized on an avidin-coated surface, the protein orientation is uniform because the position of the Avi tag in the protein is precisely controlled.
Restrictions For Research Use only
Format Lyophilized
Reconstitution For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Buffer PBS, pH 7.4
Storage -20 °C
Supplier Images
SDS-PAGE (SDS) image for Tumor Necrosis Factor Receptor Superfamily, Member 1A (TNFRSF1A) (Active) protein (AVI tag,His tag,Biotin) (ABIN3137666) Biotinylated Human TNFR1, His Tag on SDS-PAGE under reducing (R) condition. The gel w...
Binding Studies (Bind) image for Tumor Necrosis Factor Receptor Superfamily, Member 1A (TNFRSF1A) (Active) protein (AVI tag,His tag,Biotin) (ABIN3137666) Immobilized Human TNF-alpha, Tag Free with a linear range of 2.4-78 ng/mL.