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  • 产品名称：CytotoxicT-Lymphocyte-AssociatedProtein4(CTLA4)(Active)protein(FcTag,AVItag,Bio

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  • 产品厂商：ACROBiosystems
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简单介绍：
CytotoxicT-Lymphocyte-AssociatedProtein4(CTLA4)(Active)protein(FcTag,AVItag,Biotin)
详情介绍：
Brand MABSol®,PrecisionAvi
Specificity Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
Characteristics This protein carries a human IgG1 Fc fragment at the C-terminus, followed by an Avi tag (Avitag™). The protein has a calculated MW of 42 kDa. The protein migrates as 50-55 kDa on a SDS-PAGE gel under reducing (R) condition due to glycosylation.
Purity >95 % as determined by reduced SDS-PAGE.
Endotoxin Level Less than 1.0 EU per μg by the LAL method.
Background CTLA-4 (Cytotoxic T-Lymphocyte Antigen 4) is also known as CD152 (Cluster of differentiation 152), is a protein receptor that downregulates the immune system. CTLA4 is a member of the immunoglobulin superfamily, which is expressed on the surface of Helper T cells and transmits an inhibitory signal to T cells. The protein contains an extracellular V domain, a transmembrane domain, and a cytoplasmic tail. Alternate splice variants, encoding different isoforms. CTLA4 is similar to the T-cell co-stimulatory protein, CD28, and both molecules bind to CD80 and CD86, also called B7-1 and B7-2 respectively, on antigen-presenting cells. CTLA4 transmits an inhibitory signal to T cells, whereas CD28 transmits a stimulatory signal. Intracellular CTLA4 is also found in regulatory T cells and may be important to their function. Fusion proteins of CTLA4 and antibodies (CTLA4-Ig) have been used in clinical trials for rheumatoid arthritis.
Molecular Weight 42 kDa
NCBI Accession NP_005205
UniProt P16410
Comment

Ready-to-use AvitagTM biotinylated protein:
The product is exclusively produced using the AvitagTM technology. Briefly, a unique 15 amino acid peptide, the Avi tag, is introduced into the recombinant protein during expression vector construction. The single lysine residue in the Avi tag is enzymatically biotinylated by the E. Coli biotin ligase BirA.

This single-point enzymatic labeling technique brings many advantages for commonly used binding assays. The biotinylation happens on the lysine residue of Avi tag, and therefore does NOT interfere with the target protein's natural binding activities. In addition, when immobilized on an avidin-coated surface, the protein orientation is uniform because the position of the Avi tag in the protein is precisely controlled.
Restrictions For Research Use only
Format Lyophilized
Reconstitution Please see Certificate of Analysis for specific instructions. For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Buffer 50 mM Tris, 100 mM Glycine, pH 7.5
Storage -20 °C
Supplier Images
SDS-PAGE (SDS) image for Cytotoxic T-Lymphocyte-Associated Protein 4 (CTLA4) (Active) protein (Fc Tag,AVI tag,Biotin) (ABIN2870534) Biotinylated Human CTLA-4, Fc tag on SDS-PAGE under reducing (R) condition. The gel w...
Binding Studies (Bind) image for Cytotoxic T-Lymphocyte-Associated Protein 4 (CTLA4) (Active) protein (Fc Tag,AVI tag,Biotin) (ABIN2870534) Immobilized Human B7-1, Fc Tag (Cat# B71-H5259) at 5 μg/mL (100 µl/well),can bind Bio...
Binding Studies (Bind) image for Cytotoxic T-Lymphocyte-Associated Protein 4 (CTLA4) (Active) protein (Fc Tag,AVI tag,Biotin) (ABIN2870534) Immobilized Human B7-2, Fc Tag (Cat# CD6-H5257) at 5 μg/mL (100 µl/well),can bind Bio...