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  • 产品名称：KinaseinsertDomainReceptor(ATypeIIIReceptortyrosineKinase)(KDR)(Active)protein(

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  • 产品厂商：ACROBiosystems
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简单介绍：
KinaseinsertDomainReceptor(ATypeIIIReceptortyrosineKinase)(KDR)(Active)protein(Histag)
详情介绍：
Characteristics This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 86.2 kDa. The protein migrates as 100-110 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Purity >95 % as determined by SDS-PAGE.
Endotoxin Level Less than 1.0 EU per μg by the LAL method.
Grade HPLC verified
ProductDetails: Biological Activity Comment Measured by its ability to inhibit the VEGF dependent proliferation of HUVEC human umbilical vein endothelial cells. The ED50 for this effect is typically 9-20 ng/mL in the presence of 5 ng/mL rhVEGF165. Measured by its ability to bind human VEGF165 (Cat# VE5-H4210) in the SPR assay (Biacore 2000) with an estimated KD < 50nM.
Background Kinase insert domain receptor (KDR) is also known as CD309, FLK1, VEGFR, VEGFR2, and is one of the subtypes of VEGFR. VEGF receptors are receptors for vascular endothelial growth factor (VEGF). There are three main subtypes of VEGFR, numbered 1, 2 and 3. The VEGF receptors have an extracellular portion consisting of 7 immunoglobulin-like domains, a single transmembrane spanning region and an intracellular portion containing a split tyrosine-kinase domain. VEGF-A binds to VEGFR-1 (Flt-1) and VEGFR-2 (KDR/Flk-1). VEGFR-2 appears to mediate almost all of the known cellular responses to VEGF.The function of VEGFR-1 is less well defined, although it is thought to modulate VEGFR-2 signaling. Another function of VEGFR-1 may be to act as a dummy/decoy receptor, sequestering VEGF from VEGFR-2 binding (this appears to be particularly important during vasculogenesis in the embryo). In addition, VEGFR2 is able to interact with HIV-1 extracellular Tat protein upon VEGF activation, and seems to enhance angiogenesis in Kaposi's sarcoma lesions.
Molecular Weight 86.2 kDa
UniProt P35968
Pathways RTK Signaling, Glycosaminoglycan Metabolic Process, Signaling Events mediated by VEGFR1 and VEGFR2, Growth Factor Binding, Regulation of long-term Neuronal Synaptic Plasticity
Restrictions For Research Use only
Format Lyophilized
Reconstitution Please see Certificate of Analysis for specific instructions. For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Buffer PBS, pH 7.4
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment Lyophilized Protein should be stored at -20 °C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20 °C or -70 °C. Avoid repeated freeze-thaw cycles.
Supplier Images
SDS-PAGE (SDS) image for Kinase insert Domain Receptor (A Type III Receptor tyrosine Kinase) (KDR) (Active) protein (His tag) (ABIN2181918) Kinase insert Domain Receptor (A Type III Receptor tyrosine Kinase) (KDR) (Active) protein (His tag)
SDS-PAGE (SDS) image for Kinase insert Domain Receptor (A Type III Receptor tyrosine Kinase) (KDR) (Active) protein (His tag) (ABIN2181918) Human VEGF R2, His Tag (HPLC-verified) on SDS-PAGE under reducing (R) condition. The ...