产品详情
查看大图

  • 产品名称：CD3E&CD3G(AA23-126)(Active)protein(FcTag,DYKDDDDKTag,Histag)

  • 产品型号：
  • 产品厂商：ACROBiosystems
  • 产品文档：
你添加了1件商品 查看购物车
简单介绍：
CD3E&CD3G(AA23-126)(Active)protein(FcTag,DYKDDDDKTag,Histag)
详情介绍：
Characteristics The heterodimer Human CD3 epsilon & CD3 gamma is produced by co-expression of CD3E and CD3G, has a calculated MW of 39.2 kDa (CD3E) and 38.6 kDa (CD3D). Subunit CD3E is fused with a human IgG1 Fc tag at the C-terminus, followed by a polyhistidine tag, and subunit CD3G is fused with a human IgG1 Fc tag at the C-terminus, followed by a Flag tag. The predicted N-terminus is Asp 23 (CD3E) & Gln 23 (CD3G). The reducing (R) heterodimer protein migrates as 40-50 kDa due to glycosylation.
Purity >95 % as determined by SDS-PAGE.
Endotoxin Level Less than 1.0 EU per μg by the LAL method.
Background T-cell surface glycoprotein CD3 delta & CD3 gamma chain, also known as CD3D & CD3G or CD3D&CD3G respectively, are single-pass type I membrane proteins. CD3D, together with CD3- epsilon(CD3E) , CD3-gamma and CD3-zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T cell receptor-CD3 complex. T cell receptor-CD3 complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways.
Molecular Weight 39.2 kDa (CD3E) and 38.6 kDa (CD3G)
Restrictions For Research Use only
Format Lyophilized
Reconstitution Please see Certificate of Analysis for specific instructions. For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Buffer PBS, pH 7.4
Storage -20 °C
Supplier Images
SDS-PAGE (SDS) image for CD3E & CD3G (AA 23-126) (Active) protein (Fc Tag,DYKDDDDK Tag,His tag) (ABIN4949087) Human CD3 epsilon & CD3 gamma on SDS-PAGE under reducing (R) condition. The gel was s...
Binding Studies (Bind) image for CD3E & CD3G (AA 23-126) (Active) protein (Fc Tag,DYKDDDDK Tag,His tag) (ABIN4949087) Immobilized Anti-CD3E & CD3G MAb, Mouse IgG2a at 10μg/mL (100 μL/well) can bind Human...