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  • 产品名称：EctonucleotidePyrophosphatase/phosphodiesterase2(ENPP2)(Active)protein(Histag)

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  • 产品厂商：ACROBiosystems
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简单介绍：
EctonucleotidePyrophosphatase/phosphodiesterase2(ENPP2)(Active)protein(Histag)
详情介绍：
Characteristics This protein carries a polyhistidine tag at the N-terminus. The protein has a calculated MW of 94.5 kDa. The protein migrates as 120-130 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Purity >92 % as determined by SDS-PAGE.
Endotoxin Level Less than 1.0 EU per μg by the LAL method.
ProductDetails: Biological Activity Comment Measured by its ability to cleave Bis (p-Nitrophenyl) Phosphate (BPNPP). The specific activity is >8000 pmol/min/μg.
Background Autotaxin also known as ectonucleotide pyrophosphatase / phosphodiesterase family member 2 (E-NPP 2 or ENPP2), Extracellular lysophospholipase D (LysoPLD), Autotaxin (ATX or ATX-X), which belongs to the nucleotide pyrophosphatase / phosphodiesterase family. ENPP2 contains two SMB (somatomedin-B) domains. ENPP2 hydrolyzes lysophospholipids to produce lysophosphatidic acid (LPA) in extracellular fluids. ENPP2 can act on sphingosylphosphphorylcholine producing sphingosine-1-phosphate, a modulator of cell motility. ENPP2 stimulates migration of melanoma cells, probably via a pertussis toxin-sensitive G protein.
Molecular Weight 94.5 kDa
NCBI Accession NP_001035181
UniProt Q13822
Restrictions For Research Use only
Format Lyophilized
Reconstitution Please see Certificate of Analysis for specific instructions. For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Buffer 50 mM Tris, 150 mM NaCl, pH 7.5
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment Lyophilized Protein should be stored at -20 °C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20 °C or -70 °C. Avoid repeated freeze-thaw cycles.
Supplier Images
SDS-PAGE (SDS) image for Ectonucleotide Pyrophosphatase/phosphodiesterase 2 (ENPP2) (Active) protein (His tag) (ABIN2181007) Human ENPP-2, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained o...
Background publications Hausmann, Kamtekar, Christodoulou, Day, Wu, Fulkerson, Albers, van Meeteren, Houben, van Zeijl, Jansen, Andries, Hall, Pegg, Benson, Kasiem, Harlos, Kooi, Smyth, Ovaa, Bollen, Morris, Moolenaar et al.: "Structural basis of substrate discrimination and integrin binding by autotaxin. ..." in: Nature structural & molecular biology, Vol. 18, Issue 2, pp. 198-204, 2011 (PubMed).

Hausmann, Kamtekar, Christodoulou, Day, Wu, Fulkerson, Albers, van Meeteren, Houben, van Zeijl, Jansen, Andries, Hall, Pegg, Benson, Kasiem, Harlos, Kooi, Smyth, Ovaa, Bollen, Morris, Moolenaar et al.: "Structural basis of substrate discrimination and integrin binding by autotaxin. ..." in: Nature structural & molecular biology, Vol. 18, Issue 2, pp. 198-204, 2011 (PubMed).

Nam, Clair, Kim, McMarlin, Schiffmann, Liotta, Stracke: "Autotaxin (NPP-2), a metastasis-enhancing motogen, is an angiogenic factor." in: Cancer research, Vol. 61, Issue 18, pp. 6938-44, 2001 (PubMed).

Nam, Clair, Kim, McMarlin, Schiffmann, Liotta, Stracke: "Autotaxin (NPP-2), a metastasis-enhancing motogen, is an angiogenic factor." in: Cancer research, Vol. 61, Issue 18, pp. 6938-44, 2001 (PubMed).