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  • 产品名称：LeukemiaInhibitoryFactorReceptoralpha(LIFR)(Active)protein(FcTag)

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  • 产品厂商：ACROBiosystems
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简单介绍：
LeukemiaInhibitoryFactorReceptoralpha(LIFR)(Active)protein(FcTag)
详情介绍：
Characteristics This protein carries a human IgG1 Fc tag at the C-terminus. The protein has a calculated MW of 115.5 kDa. The protein migrates as 135-150 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Purity >95 % as determined by SDS-PAGE.
Endotoxin Level Less than 1.0 EU per μg by the LAL method.
ProductDetails: Biological Activity Comment Measured by its ability to inhibit LIF-dependent proliferation of TF1 human erythroleukemic cells. The ED50 for this effect is typically 5-10 μg/ml in the presence of 0. 3 ng/mL of recombinant human LIF.
Background Leukemia inhibitory factor receptor is also known as LIFR, CD118, FLJ98106, FLJ99923, LIF-R, SJS2, STWS, SWS, is the receptor for leukemia inhibitory factor (LIF). The leukemia inhibitory factor is a polyfunctional cytokine that affects the differentiation, survival, and proliferation of a wide variety of cells in the ***** and the embryo. LIF action appears to be mediated through a high-affinity receptor complex composed of a low-affinity LIF binding chain (LIF receptor) and a high-affinity converter subunit, gp130. Both LIFR and gp130 are members of a family of cytokine receptors that includes components of the receptors for the majority of hematopoietic cytokines and for cytokines that affect other systems, including the ciliary neurotrophic factor, growth hormone and prolactin Defects in LIFR are the cause of Stueve-Wiedemann syndrome (SWS), a severe autosomal recessive condition and belongs to the group of the bent-bone dysplasias.
Molecular Weight 115.5 kDa
NCBI Accession NP_001121143
UniProt P42702
Pathways JAK-STAT Signaling, Growth Factor Binding
Restrictions For Research Use only
Format Lyophilized
Reconstitution Please see Certificate of Analysis for specific instructions. For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Buffer 50 mM Tris, 100 mM Glycine, pH 7.5
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment Lyophilized Protein should be stored at -20 °C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20 °C or -70 °C. Avoid repeated freeze-thaw cycles.
Supplier Images
SDS-PAGE (SDS) image for Leukemia Inhibitory Factor Receptor alpha (LIFR) (Active) protein (Fc Tag) (ABIN2444162) Leukemia Inhibitory Factor Receptor alpha (LIFR) (Active) protein (Fc Tag)
Binding Studies (Bind) image for Leukemia Inhibitory Factor Receptor alpha (LIFR) (Active) protein (Fc Tag) (ABIN2444162) Immobilized Human LIF, Tag Free with a linear range of 7.8-31.25 ng/mL.
Background publications Timmermann, Küster, Kurth, Heinrich, Müller-Newen: "A functional role of the membrane-proximal extracellular domains of the signal transducer gp130 in heterodimerization with the leukemia inhibitory factor receptor." in: European journal of biochemistry / FEBS, Vol. 269, Issue 11, pp. 2716-26, 2002 (PubMed).

Timmermann, Küster, Kurth, Heinrich, Müller-Newen: "A functional role of the membrane-proximal extracellular domains of the signal transducer gp130 in heterodimerization with the leukemia inhibitory factor receptor." in: European journal of biochemistry / FEBS, Vol. 269, Issue 11, pp. 2716-26, 2002 (PubMed).

Mosley, De Imus, Friend, Boiani, Thoma, Park, Cosman: "Dual oncostatin M (OSM) receptors. Cloning and characterization of an alternative signaling subunit conferring OSM-specific receptor activation." in: The Journal of biological chemistry, Vol. 271, Issue 51, pp. 32635-43, 1997 (PubMed).

Mosley, De Imus, Friend, Boiani, Thoma, Park, Cosman: "Dual oncostatin M (OSM) receptors. Cloning and characterization of an alternative signaling subunit conferring OSM-specific receptor activation." in: The Journal of biological chemistry, Vol. 271, Issue 51, pp. 32635-43, 1997 (PubMed).

Gearing, Thut, VandeBos, Gimpel, Delaney, King, Price, Cosman, Beckmann: "Leukemia inhibitory factor receptor is structurally related to the IL-6 signal transducer, gp130." in: The EMBO journal, Vol. 10, Issue 10, pp. 2839-48, 1991 (PubMed).

Gearing, Thut, VandeBos, Gimpel, Delaney, King, Price, Cosman, Beckmann: "Leukemia inhibitory factor receptor is structurally related to the IL-6 signal transducer, gp130." in: The EMBO journal, Vol. 10, Issue 10, pp. 2839-48, 1991 (PubMed).