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  • 产品名称：CD28(CD28)(AA19-152)(Active)protein(FcTag,AVItag,Biotin)

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  • 产品厂商：ACROBiosystems
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简单介绍：
CD28(CD28)(AA19-152)(Active)protein(FcTag,AVItag,Biotin)
详情介绍：
Brand MABSol®,PrecisionAvi
Specificity Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
Characteristics This protein carries a human IgG1 Fc tag at the C-terminus, followed by a Avi tag (Avitag™). The protein has a calculated MW of 43.6 kDa. As a result of glycosylation, the protein migrates as 55-66 kDa under reducing (R) condition, and 110-130 kDa under non-reducing (NR) condition (SDS-PAGE).
Purity >95 % as determined by SDS-PAGE.
Endotoxin Level Less than 1.0 EU per μg by the LAL method.
Background T-cell-specific surface glycoprotein CD28 is also known as TP44, is a single-pass type I membrane protein which contains one Ig-like V-type (immunoglobulin-like) domain. is one of the molecules expressed on T cells that provide co-stimulatory signals, which are required for T cell activation. CD28 is the receptor for CD80 (B7.1) and CD86 (B7.2). When activated by Toll-like receptor ligands, the CD80 expression is upregulated in antigen presenting cells (APCs). The CD86 expression on antigen presenting cells is constitutive. CD28 is the only B7 receptor constitutively expressed on naive T cells.
Molecular Weight 43.6 kDa
UniProt P10747
Research Area Surface Receptors of Immune Cells, Adaptive Immunity, Hematopoietic Progenitors, CD Antigens, Apoptosis/Necrosis, Immunology
Pathways TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response
Comment

Ready-to-use AvitagTM biotinylated protein:
The product is exclusively produced using the AvitagTM technology. Briefly, a unique 15 amino acid peptide, the Avi tag, is introduced into the recombinant protein during expression vector construction. The single lysine residue in the Avi tag is enzymatically biotinylated by the E. Coli biotin ligase BirA.

This single-point enzymatic labeling technique brings many advantages for commonly used binding assays. The biotinylation happens on the lysine residue of Avi tag, and therefore does NOT interfere with the target protein's natural binding activities. In addition, when immobilized on an avidin-coated surface, the protein orientation is uniform because the position of the Avi tag in the protein is precisely controlled.
Restrictions For Research Use only
Format Lyophilized
Reconstitution Please see Certificate of Analysis for specific instructions. For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Buffer 50 mM Tris, 100 mM Glycine, pH 7.5
Storage -20 °C
Supplier Images
SDS-PAGE (SDS) image for CD28 (CD28) (AA 19-152) (Active) protein (Fc Tag,AVI tag,Biotin) (ABIN3137669) Biotinylated Human CD28, Fc Tag on SDS-PAGE under reducing (R) and no-reducing (NR) c...
Binding Studies (Bind) image for CD28 (CD28) (AA 19-152) (Active) protein (Fc Tag,AVI tag,Biotin) (ABIN3137669) Immobilized Anti-CD28 MAb, Mouse IgG1 at 5μg/mL (100 μL/well) can bind Biotinylated H...
Binding Studies (Bind) image for CD28 (CD28) (AA 19-152) (Active) protein (Fc Tag,AVI tag,Biotin) (ABIN3137669) Immobilized Human B7-2, Fc Tag with a linear range of 0.15-5 μg/mL.