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  • 产品名称：V-Erb-B2erythroblasticLeukemiaViralOncogeneHomolog2,Neuro/glioblastomaDerivedOn

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  • 产品厂商：ACROBiosystems
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简单介绍：
V-Erb-B2erythroblasticLeukemiaViralOncogeneHomolog2,Neuro/glioblastomaDerivedOncogeneHomolog(Avian)(ERBB2)(Active)protein(Biotin,Histag)
详情介绍：
Brand MABSol®,UltraLys
Specificity The primary amines in the side chains of lysine residues and the N-terminus of the protein are conjugated with biotins using standard chemical labeling method. A standard biotin reagent (13.5 angstroms) is used in this product.
Characteristics This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 70.2 kDa. The protein migrates as 110-115 kDa on a SDS-PAGE gel under reducing (R) condition due to glycosylation.
Purity >95 % as determined by reduced SDS-PAGE.
Endotoxin Level Less than 1.0 EU per μg by the LAL method.
Grade Ultra Sensitive
ProductDetails: Biological Activity Comment Measured by its binding ability in a functional ELISA. Immobilized Herceptin mAb at 3 μg/mL (100 μl/well) can bind Biotinylated Human HER2 /ErbB2 Protein, Superior Sensitivity (Catalog # HE2-H822R). The EC50 of Biotinylated human HER2 is 0. 09-1 ng/mL.
Background Human Epidermal growth factor Receptor 2 (HER2),also called ERBB2, HER-2,HER-2/neu, NEU, NGL,TKR1 and c-erb B2,and is a protein giving higher aggressiveness in breast cancers. It is a member of the ErbB protein family, more commonly known as the epidermal growth factor receptor family. HER2 is a cell membrane surface-bound receptor tyrosine kinase and is normally involved in the signal transduction pathways leading to cell growth and differentiation. HER2 is thought to be an orphan receptor, with none of the EGF family of ligands able to activate it. Approximately 30 % of breast cancers have an amplification of the HER2 gene or overexpression of its protein product. Overexpression of this receptor in breast cancer is associated with increased disease recurrence and worse prognosis. HER2 appears to play roles in development, cancer,communication at the neuromuscular junction andregulation of cell growth and differentiation.
Molecular Weight 70.2 kDa
UniProt P04626
Pathways RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Skeletal Muscle Fiber Development
Comment

A chemically labeled biotinylated protein with ultra sensitivity.
The product is produced using a chemical labeling approach. The primary amines in the side chains of lysine residues and the N-terminus of protein are conjugated with biotins.
Chemical labeling usually results in multiple biotin attachment on a single protein molecule, which could potentially lead to higher detection sensitivity.
Restrictions For Research Use only
Format Lyophilized
Reconstitution Please see Certificate of Analysis for specific instructions. For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Buffer PBS, pH 7.4
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment Lyophilized Protein should be stored at -20 °C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20 °C or -70 °C. Avoid repeated freeze-thaw cycles.
Supplier Images
SDS-PAGE (SDS) image for V-Erb-B2 erythroblastic Leukemia Viral Oncogene Homolog 2, Neuro/glioblastoma Derived Oncogene Homolog (Avian) (ERBB2) (Active) protein (Biotin,His tag) (ABIN2444140) Biotinylated Human Her2, Superior Sensitivity on SDS-PAGE under reducing (R) conditio...
Binding Studies (Bind) image for V-Erb-B2 erythroblastic Leukemia Viral Oncogene Homolog 2, Neuro/glioblastoma Derived Oncogene Homolog (Avian) (ERBB2) (Active) protein (Biotin,His tag) (ABIN2444140) Immobilized trastuzumab at 3 μg/mL (100 μl/well) can bind Biotinylated Human Her2, Su...