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  • 产品名称：CarbonicAnhydraseX(CA10)(AA22-300)(Active)protein(Histag)

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  • 产品厂商：ACROBiosystems
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简单介绍：
CarbonicAnhydraseX(CA10)(AA22-300)(Active)protein(Histag)
详情介绍：
Characteristics This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 32.6 kDa. The protein migrates as 37 kDa on a SDS-PAGE gel under reducing (R) condition due to glycosylation.
Purity >95 % as determined by SDS-PAGE.
Sterility 0.22 μm filtered
Endotoxin Level Less than 1.0 EU per μg by the LAL method.
ProductDetails: Biological Activity Comment Biological Activity: Measured by its esterase activity. The specific activity, as measured with 1 mM 4-Nitrophenyl acetate (4-NPA) (Sigma, Catalog # N8130) as substrate and 5 μg rh CA10 / CARPX enzyme at 100 ng/μL in 100 μL of 12.5 mM Tris, 75 mM NaCl, pH 7.5. The specific activity is >1 pmol/min/μg.
Background Carbonic anhydrase-related protein 10 (CA10) is also known as Carbonic anhydrase-related protein X (CARP X or CARPX), Cerebral protein 15 (hucep-15), which belongs to the alpha-carbonic anhydrase family of zinc metalloenzymes, which catalyze the reversible hydration of carbon dioxide in various biological processes. CA10 / CARPX is an acatalytic member of the alpha-carbonic anhydrase subgroup, and it is thought to play a role in the central nervous system, especially in brain development.
Molecular Weight 32.6 kDa
UniProt Q9NS85
Restrictions For Research Use only
Format Lyophilized
Reconstitution For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Buffer 50 mM Tris, 150 mM NaCl, pH 7.5
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment No activity loss was observed after storage at: In lyophilized state for 1 year (4 °C), After reconstitution under sterile conditions for 3 months (-70 °C).
Supplier Images
SDS-PAGE (SDS) image for Carbonic Anhydrase X (CA10) (AA 22-300) (Active) protein (His tag) (ABIN2180668) Carbonic Anhydrase X (CA10) (AA 22-300) (Active) protein (His tag)