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  • 产品名称：Interleukin7Receptor(IL7R)(AA21-236)(Active)protein(FcTag,AVItag,Biotin)

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  • 产品厂商：ACROBiosystems
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简单介绍：
Interleukin7Receptor(IL7R)(AA21-236)(Active)protein(FcTag,AVItag,Biotin)
详情介绍：
Brand MABSol®,PrecisionAvi
Specificity Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
Characteristics This protein carries a human IgG1 Fc tag at the C-terminus, followed by a Avi tag (Avitag™). The protein has a calculated MW of 53.3 kDa. As a result of glycosylation, the protein migrates as 70-100 kDa under reducing (R) condition, and 130-200 kDa under non-reducing (NR) condition (SDS-PAGE).
Purity >95 % as determined by SDS-PAGE.
Endotoxin Level Less than 1.0 EU per μg by the LAL method.
Background Interleukin-7 receptor subunit alpha (IL-7 RA or IL7Ra) is also known as cluster of differentiation 127 (CD127). IL-7 RA is a type I cytokine receptor and is a subunit of the functional Interleukin-7 receptor and thymic stromal lymphopoietin (TSLP) receptors. IL-7 RA has been shown to play a critical role in the development of immune cells called lymphocytes - specifically in a process known as V(D)J recombination[citation needed]. This protein is also found to control the accessibility of a region of the genome that contains the T-cell receptor gamma gene, by STAT5 and histone acetylation.
Molecular Weight 53.3 kDa
UniProt P16871
Pathways JAK-STAT Signaling, Regulation of Leukocyte Mediated Immunity, Production of Molecular Mediator of Immune Response, Regulation of Cell Size
Comment

Ready-to-use AvitagTM biotinylated protein:
The product is exclusively produced using the AvitagTM technology. Briefly, a unique 15 amino acid peptide, the Avi tag, is introduced into the recombinant protein during expression vector construction. The single lysine residue in the Avi tag is enzymatically biotinylated by the E. Coli biotin ligase BirA.

This single-point enzymatic labeling technique brings many advantages for commonly used binding assays. The biotinylation happens on the lysine residue of Avi tag, and therefore does NOT interfere with the target protein's natural binding activities. In addition, when immobilized on an avidin-coated surface, the protein orientation is uniform because the position of the Avi tag in the protein is precisely controlled.
Restrictions For Research Use only
Format Lyophilized
Reconstitution Please see Certificate of Analysis for specific instructions. For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Buffer 50 mM Tris, 100 mM Glycine, pH 7.5
Storage -20 °C
Supplier Images
SDS-PAGE (SDS) image for Interleukin 7 Receptor (IL7R) (AA 21-236) (Active) protein (Fc Tag,AVI tag,Biotin) (ABIN4949034) Biotinylated Human IL-7 RA / CD127, Fc Tag on SDS-PAGE under reducing (R) and no-redu...
image for Interleukin 7 Receptor (IL7R) (AA 21-236) (Active) protein (Fc Tag,AVI tag,Biotin) (ABIN4949034) Measured by its binding ability in a functional ELISA. Immobilized Human IL-7, Tag Fr...