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  • 产品名称：BandTLymphocyteAssociated(BTLA)(AA31-134)(Active)protein(FcTag,AVItag,Biotin)

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  • 产品厂商：ACROBiosystems
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简单介绍：
BandTLymphocyteAssociated(BTLA)(AA31-134)(Active)protein(FcTag,AVItag,Biotin)
详情介绍：
Brand MABSol®,PrecisionAvi
Specificity Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
Characteristics This protein carries a human IgG1 Fc tag at the C-terminus, followed by a Avi tag (Avitag™). The protein has a calculated MW of 40.5 kDa. The protein migrates as 55-66 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Purity >95 % as determined by SDS-PAGE.
Endotoxin Level Less than 1.0 EU per μg by the LAL method.
Background B- and T-lymphocyte attenuator (BTLA) is also known as B- and T-lymphocyte-associated protein, CD antigen CD272. BTLA contains one Ig-like V-type (immunoglobulin-like) domain. As a lymphocyte inhibitory receptor, BTLA / CD272 inhibits lymphocytes during immune response. BTLA / CD272 can interact with tyrosine phosphatases PTPN6/SHP-1 and PTPN11/SHP-2, and interact with TNFRSF14/HVEM.
Molecular Weight 40.5 kDa
UniProt Q7Z6A9
Comment

Ready-to-use AvitagTM biotinylated protein:
The product is exclusively produced using the AvitagTM technology. Briefly, a unique 15 amino acid peptide, the Avi tag, is introduced into the recombinant protein during expression vector construction. The single lysine residue in the Avi tag is enzymatically biotinylated by the E. Coli biotin ligase BirA.

This single-point enzymatic labeling technique brings many advantages for commonly used binding assays. The biotinylation happens on the lysine residue of Avi tag, and therefore does NOT interfere with the target protein's natural binding activities. In addition, when immobilized on an avidin-coated surface, the protein orientation is uniform because the position of the Avi tag in the protein is precisely controlled.
Restrictions For Research Use only
Format Lyophilized
Reconstitution Please see Certificate of Analysis for specific instructions. For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Buffer 50 mM Tris, 100 mM Glycine, pH 7.5
Storage -20 °C
Supplier Images
SDS-PAGE (SDS) image for B and T Lymphocyte Associated (BTLA) (AA 31-134) (Active) protein (Fc Tag,AVI tag,Biotin) (ABIN4949010) Biotinylated Human BTLA (31-134), Fc Tag on SDS-PAGE under reducing (R) condition. Th...
image for B and T Lymphocyte Associated (BTLA) (AA 31-134) (Active) protein (Fc Tag,AVI tag,Biotin) (ABIN4949010) Measured by its binding ability in a functional ELISA. Immobilized Human HVEM, Fc Tag...