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  • 产品名称：ProproteinConvertaseSubtilisin/kexinType9(PCSK9)(Asp374Tyr-Mutation)(Active)pro

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  • 产品厂商：ACROBiosystems
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简单介绍：
ProproteinConvertaseSubtilisin/kexinType9(PCSK9)(Asp374Tyr-Mutation)(Active)protein(AVItag,Biotin,Histag)
详情介绍：
Brand MABSol®,PrecisionAvi
Specificity Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
Characteristics This protein carries an Avi tag (Avitag™) at the C-terminus, followed by a polyhistidine tag. The protein has a calculated MW of 73.7 kDa. The protein migrates as 16 kDa,18 kDa and 65 kDa on a SDS-PAGE gel under reducing (R) condition due to glycosylation and proteolytic digestion.
Purity >95 % as determined by reduced SDS-PAGE.
Endotoxin Level Less than 1.0 EU per μg by the LAL method.
Background Proprotein convertase subtilisin/kexin type 9 (PCSK9), is an enzyme which in humans is encoded by the PCSK9 gene. This gene encodes a proprotein convertase belonging to the proteinase K subfamily of the secretory subtilase family. This protein plays a major regulatory role in cholesterol homeostasis. PCSK9 binds to the epidermal growth factor-like repeat A (EGF-A) domain of the low-density lipoprotein receptor (LDLR), inducing LDLR degradation. PCSK9 may also have a role in the differentiation of cortical neurons. Mutations in this gene have been associated with a rare form of autosomal dominant familial hypercholesterolemia (HCHOLA3).Especially,the D374Y mutation results in higher affinity of PCSK9 for LDLR.
Molecular Weight 73.7 kDa
UniProt Q8NBP7
Research Area Proteolysis / Ubiquitin, Metabolism, Amino Acids
Comment

Ready-to-use AvitagTM biotinylated protein:
The product is exclusively produced using the AvitagTM technology. Briefly, a unique 15 amino acid peptide, the Avi tag, is introduced into the recombinant protein during expression vector construction. The single lysine residue in the Avi tag is enzymatically biotinylated by the E. Coli biotin ligase BirA.

This single-point enzymatic labeling technique brings many advantages for commonly used binding assays. The biotinylation happens on the lysine residue of Avi tag, and therefore does NOT interfere with the target protein's natural binding activities. In addition, when immobilized on an avidin-coated surface, the protein orientation is uniform because the position of the Avi tag in the protein is precisely controlled.
Restrictions For Research Use only
Format Lyophilized
Reconstitution Please see Certificate of Analysis for specific instructions. For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Buffer PBS, pH 7.4
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment Lyophilized Protein should be stored at -20 °C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20 °C or -70 °C. Avoid repeated freeze-thaw cycles.
Supplier Images
SDS-PAGE (SDS) image for Proprotein Convertase Subtilisin/kexin Type 9 (PCSK9) (Asp374Tyr-Mutation) (Active) protein (AVI tag,Biotin,His tag) (ABIN2444180) Biotinylated Human (D374Y) PCSK9 on SDS-PAGE under reducing (R) condition. The gel wa...
Binding Studies (Bind) image for Proprotein Convertase Subtilisin/kexin Type 9 (PCSK9) (Asp374Tyr-Mutation) (Active) protein (AVI tag,Biotin,His tag) (ABIN2444180) Immobilized Human LDL R, His Tag (Cat# LDR-H5224) at 10 μg/mL (100 μl/well) can bind ...