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  • 产品名称：EpithelialCellAdhesionMolecule(EPCAM)(Active)protein(Biotin,Histag)

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  • 产品厂商：ACROBiosystems
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简单介绍：
EpithelialCellAdhesionMolecule(EPCAM)(Active)protein(Biotin,Histag)
详情介绍：
Brand MABSol®,UltraLys
Specificity The primary amines in the side chains of lysine residues and the N-terminus of the protein are conjugated with biotins using standard chemical labeling method. A standard biotin reagent (13.5 angstroms) is used in this product.
Characteristics This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 29 kDa. The protein migrates as 33-36 kDa on a SDS-PAGE gel under reducing (R) condition due to glycosylation.
Purity >95 % as determined by reduced SDS-PAGE.
Endotoxin Level Less than 1.0 EU per μg by the LAL method.
Grade Ultra Sensitive
Background EpCAM is also known as CO171A, EGP, EGP40, GA7332, KSA, M4S, MIC18, MK1, TROP1, hEGP2, and is a pan-epithelial differentiation antigen that is expressed on almost all carcinomas as 17-1A(mAb) antigen. Its constitutional function is being elucidated. It is intricately linked with the Cadherin-Catenin pathway and hence the fundamental WNT pathway responsible for intracellular signaling and polarity. The epithelial cell adhesion molecule (Ep-CAM) is known to express in most epithelial malignancies and was reported as a tumor marker or a candidate of molecular targeting therapy. Ep-CAM cross signaling with N-cadherin involves Pi3K, resulting in the abrogation of the cadherin adhesion complexes in epithelial cells was reported. And Epithelial cell adhesion molecule (Ep-CAM) recently received increased attention as a prognostic factor in breast cancer.
Molecular Weight 29 kDa
UniProt P16422
Research Area Cancer
Comment

A chemically labeled biotinylated protein with ultra sensitivity.
The product is produced using a chemical labeling approach. The primary amines in the side chains of lysine residues and the N-terminus of protein are conjugated with biotins.
Chemical labeling usually results in multiple biotin attachment on a single protein molecule, which could potentially lead to higher detection sensitivity.
Restrictions For Research Use only
Format Lyophilized
Reconstitution Please see Certificate of Analysis for specific instructions. For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Buffer PBS, pH 7.4
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment Lyophilized Protein should be stored at -20 °C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20 °C or -70 °C. Avoid repeated freeze-thaw cycles.
Supplier Images
SDS-PAGE (SDS) image for Epithelial Cell Adhesion Molecule (EPCAM) (Active) protein (Biotin,His tag) (ABIN2444113) Biotinylated Human EpCAM on SDS-PAGE under reducing (R) condition. The gel was staine...