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  • 产品名称：V-SetDomainContainingTCellActivationInhibitor1(VTCN1)(AA29-258)(Active)protein(

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  • 产品厂商：ACROBiosystems
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简单介绍：
V-SetDomainContainingTCellActivationInhibitor1(VTCN1)(AA29-258)(Active)protein(AVItag,Biotin,Histag)
详情介绍：
Brand MABSol®,PrecisionAvi
Specificity Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
Characteristics This protein carries an Avi tag (Avitag™) at the C-terminus, followed by a polyhistidine tag. The protein has a calculated MW of 28 kDa. The protein migrates as 45-65 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Purity >90 % as determined by SDS-PAGE.
Endotoxin Level Less than 1.0 EU per μg by the LAL method.
Background V-set domain-containing T-cell activation inhibitor 1 (VTCN1) is also known as Immune costimulatory protein B7-H4, Protein B7S1, T-cell costimulatory molecule B7x, B7H4, which belongs to the immunoglobulin superfamily and BTN/MOG family. VTCN1 contains two Ig-like V-type (immunoglobulin-like) domains. The expression of VTCN1 is up-regulated by IL6 and IL10 and is inhibited by GM-CSF and IL4 on antigen-presenting cells (APCs). VTCN1 / B7-H4 negatively regulates T-cell-mediated immune response by inhibiting T-cell activation, proliferation, cytokine production and development of cytotoxicity. VTCN1 involved in promoting epithelial cell transformation.
Molecular Weight 28 kDa
NCBI Accession NP_078902
UniProt Q7Z7D3
Comment

Ready-to-use AvitagTM biotinylated protein:
The product is exclusively produced using the AvitagTM technology. Briefly, a unique 15 amino acid peptide, the Avi tag, is introduced into the recombinant protein during expression vector construction. The single lysine residue in the Avi tag is enzymatically biotinylated by the E. Coli biotin ligase BirA.

This single-point enzymatic labeling technique brings many advantages for commonly used binding assays. The biotinylation happens on the lysine residue of Avi tag, and therefore does NOT interfere with the target protein's natural binding activities. In addition, when immobilized on an avidin-coated surface, the protein orientation is uniform because the position of the Avi tag in the protein is precisely controlled.
Restrictions For Research Use only
Format Lyophilized
Reconstitution Please see Certificate of Analysis for specific instructions. For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Buffer PBS, pH 7.4
Storage -20 °C
Supplier Images
SDS-PAGE (SDS) image for V-Set Domain Containing T Cell Activation Inhibitor 1 (VTCN1) (AA 29-258) (Active) protein (AVI tag,Biotin,His tag) (ABIN4949004) Biotinylated Human B7-H4 (recommended for biopanning) on SDS-PAGE under reducing (R) ...
image for V-Set Domain Containing T Cell Activation Inhibitor 1 (VTCN1) (AA 29-258) (Active) protein (AVI tag,Biotin,His tag) (ABIN4949004) Measured by its binding ability in a functional ELISA. Immobilized Anti-B7-H4 mAb,Hum...